Cloning, expression, and chromosomal localization of the rat mitochondrial capsule selenoprotein gene (MCS): the reading frame does not contain potential UGA selenocysteine codons
| Autor: | Ibrahim M. Adham, Hannelore Kremling, Dirk Tessmann, Claude Szpirer, Derek Murphy, Wolfgang Engel, Khalid A. Soliman |
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| Rok vydání: | 1996 |
| Předmět: |
Male
DNA Complementary Molecular Sequence Data CAAT box Gene Expression Sequence alignment Biology 03 medical and health sciences chemistry.chemical_compound Mice Open Reading Frames 0302 clinical medicine Species Specificity Complementary DNA Genetics Animals Amino Acid Sequence Cloning Molecular Selenoproteins Molecular Biology Gene Peptide sequence 030304 developmental biology Gene Library 0303 health sciences Selenocysteine Base Sequence Intron Chromosome Mapping Proteins Cell Biology General Medicine Molecular biology Spermatids humanities Mitochondria Rats Open reading frame chemistry Genes 030220 oncology & carcinogenesis Protein Biosynthesis Sequence Alignment |
| Zdroj: | DNA and cell biology. 15(2) |
| ISSN: | 1044-5498 |
| Popis: | The mitochondrial capsule selenoprotein (MCS) is a selenium-containing polypeptide. It is one of three proteins that are important for the maintenance and stabilization of the crescent structure of the sperm mitochondria. In this paper, we report the isolation and characterization of the rat MCS cDNA and gene. The cDNA contains a reading frame for a 145-amino-acid protein and it lacks the UGA codons, which have been found in the reading frame of the mouse MCS cDNA and have been presumed to encode the selenocysteine in the amino terminal of the deduced mouse amino acid sequence. The deduced amino acid sequence of the rat and mouse MCS shows a high level of homology (79%). The rat MCS gene contains two exons; the intron sequence interrupts the 5' untranslated sequence at the same position as in the mouse MCS gene. The transcription start site is located 184 bp upstream of the translation start site. Alignment of the 5'-flanking regions of the mouse and rat genes reveals that the first 400 nucleotides upstream of the transcription start site exhibit an overall sequence similarity of 73%. This conserved region contains no TATA or CAAT box motifs. Northern blot analysis indicates that the MCS mRNA is detectable only in the testis after day 30 of postnatal development. Moreover, in situ hybridization revealed that the rat MCS gene is mainly expressed in round spermatids. From the analysis of mouse-rat cell hybrids that segregate rat chromosomes, the MCS gene was assigned to rat chromosome 2. |
| Databáze: | OpenAIRE |
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