Microporous calcium phosphate ceramics as tissue engineering scaffolds for the repair of osteochondral defects: Biomechanical results
| Autor: | Marc Bohner, Norbert P. Südkamp, Anke Bernstein, Philipp Niemeyer, M. Menzel, Gian M. Salzmann, J. Klehm, H. O. Mayr, R. Hube, L. Görz, R. von Eisenhart-Rothe |
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| Rok vydání: | 2013 |
| Předmět: |
Calcium Phosphates
Ceramics Materials science Biomedical Engineering Calcium phosphate ceramics Biochemistry Bone and Bones Biomaterials Tissue engineering medicine Animals Cartilage repair Molecular Biology Sheep Tissue Engineering Tissue Scaffolds Cartilage General Medicine Microporous material Biomechanical Phenomena Resorption medicine.anatomical_structure Synovial membrane Biotechnology Large animal Biomedical engineering |
| Zdroj: | Acta Biomaterialia. 9:4845-4855 |
| ISSN: | 1742-7061 |
| Popis: | This work investigated the suitability of microporous β-tricalcium phosphate (TCP) scaffolds pre-seeded with autologous chondrocytes for treatment of osteochondral defects in a large animal model. Microporous β-TCP cylinders (O 7 mm; length 25 mm) were seeded with autologous chondrocytes and cultured for 4 weeks in vitro. Only the upper end of the cylinder was seeded with chondrocytes. Chondrocytes formed a multilayer on the top. The implants were then implanted in defects (diameter 7 mm) created in the left medial femoral condyle of ovine knees. The implants were covered with synovial membrane from the superior recess of the same joint. For the right knees, an empty defect with the same dimensions served as control. Twenty-eight sheep were split into 6-, 12-, 26- and 52 week groups of seven animals. Indentation tests with a spherical (O 3mm) indenter were used to determine the biomechanical properties of regenerated tissue. A software-based limit switch was implemented to ensure a maximal penetration depth of 200 μm and maximal load of 1.5 N. The achieved load, the absorbed energy and the contact stiffness were measured. Newly formed cartilage was assessed with the International Cartilage Repair Society Visual Assessment Scale (ICRS score) and histomorphometric analysis. Results were analysed statistically using the t-test, Mann-Whitney U-test and Wilcoxon test. Statistical significance was set at p |
| Databáze: | OpenAIRE |
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