Liquid chromatographic method for separation and determination of elaiophylin in biotechnology processes of salinomycin production
| Autor: | Milenko Sokolic, Friedrich H. Johannsen, Vladimir Pokorni |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Chromatography
Column temperature business.industry Methanol Clinical Biochemistry Pharmaceutical Science Water Hydrogen phosphate Quantitative determination Analytical Chemistry Biotechnology Anti-Bacterial Agents Phosphates Elaiophylin chemistry.chemical_compound chemistry Drug Discovery Macrolides business Spectroscopy Salinomycin Chromatography High Pressure Liquid Pyrans |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 10(10-12) |
| ISSN: | 0731-7085 |
| Popis: | An LC method for the quick and precise quantitative determination of elaiophylin in biotechnology processes of salinomycin production was prepared. A mobile phase of methanol 0.04 M diammonium hydrogen phosphate pH 6.0-ethyl acetate (55:35:10, v/v/v) and an elevated column temperature optimized the chromatography. Separation of elaiophylin was obtained in 4–6 min. Positive identification and peak purity of elaiophylin have been carried out by photodiode array (PDA) detection. An agreement between the PDA spectra of samples and the elaiophylin standard were obtained. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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