Expression patterns of ERVWE1/Syncytin-1 and other placentally expressed human endogenous retroviruses along the malignant transformation process of hydatidiform moles

Autor: Pierre-Adrien Bolze, Michèle Guillotte, Sophie Patrier, Jérôme Massardier, François Golfier, Damien Sanlaville, Guy Oriol, Marc Bossus, Touria Hajri, François Mallet, Valérie Cheynet
Rok vydání: 2015
Předmět:
0301 basic medicine
medicine.medical_specialty
Transcription
Genetic
Biology
Pregnancy Proteins
Malignant transformation
Human chorionic gonadotropin
03 medical and health sciences
0302 clinical medicine
Syncytiotrophoblast
Proviruses
Pregnancy
Internal medicine
medicine
Humans
Placental site trophoblastic tumor
Promoter Regions
Genetic
reproductive and urinary physiology
Partial Hydatidiform Mole
030219 obstetrics & reproductive medicine
Gestational trophoblastic disease
Choriocarcinoma
Obstetrics and Gynecology
Gene Products
env
Promoter
Hydatidiform Mole
DNA Methylation
medicine.disease
female genital diseases and pregnancy complications
Trophoblasts
030104 developmental biology
medicine.anatomical_structure
Endocrinology
Cell Transformation
Neoplastic
Reproductive Medicine
embryonic structures
Uterine Neoplasms
Cancer research
Female
Developmental Biology
Zdroj: Placenta. 39
ISSN: 1532-3102
Popis: Introduction Up to 20% of hydatidiform moles are followed by malignant transformation in gestational trophoblastic neoplasia and require chemotherapy. Syncytin-1 is involved in human placental morphogenesis and is also expressed in various cancers. We assessed the predictive value of the expression of Syncytin-1 and its interactants in the malignant transformation process of hydatidiform moles. Methods Syncytin-1 glycoprotein was localized by immunohistochemistry in hydatidiform moles, gestational trophoblastic neoplasia and control placentas. The transcription levels of its locus ERVWE1, its interaction partners (hASCT1, hASCT2, TLR4 and DC-SIGN) and two loci (ERVFRDE1 and ERV3) involved the expression of other placental envelopes were assessed by real-time PCR. Results Syncytin-1 glycoprotein was expressed in syncytiotrophoblast of hydatidiform moles with an apical enhancement when compared with normal placentas. Moles with further malignant transformation had a higher staining intensity of Syncytin-1 surface unit C-terminus but the transcription level of its locus ERVWE1 was not different from that of moles with further remission and normal placentas. hASCT1 and TLR4, showed lower transcription levels in complete moles when compared to normal placentas. ERVWE1, ERVFRDE1 and ERV3 transcription was down-regulated in hydatidiform moles and gestational trophoblastic neoplasia. Conclusions Variations of Syncytin-1 protein localization and down-regulation of hASCT1 and TLR4 transcription are likely to reflect altered functions of Syncytin-1 in the premalignant context of complete moles. The reduced transcription in gestational trophoblastic diseases of ERVWE1, ERVFRDE1 and ERV3, which expression during normal pregnancy is differentially regulated by promoter region methylation, suggest a joint dysregulation mechanism in malignant context.
Databáze: OpenAIRE
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