Expression patterns of ERVWE1/Syncytin-1 and other placentally expressed human endogenous retroviruses along the malignant transformation process of hydatidiform moles
Autor: | Pierre-Adrien Bolze, Michèle Guillotte, Sophie Patrier, Jérôme Massardier, François Golfier, Damien Sanlaville, Guy Oriol, Marc Bossus, Touria Hajri, François Mallet, Valérie Cheynet |
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Rok vydání: | 2015 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Transcription Genetic Biology Pregnancy Proteins Malignant transformation Human chorionic gonadotropin 03 medical and health sciences 0302 clinical medicine Syncytiotrophoblast Proviruses Pregnancy Internal medicine medicine Humans Placental site trophoblastic tumor Promoter Regions Genetic reproductive and urinary physiology Partial Hydatidiform Mole 030219 obstetrics & reproductive medicine Gestational trophoblastic disease Choriocarcinoma Obstetrics and Gynecology Gene Products env Promoter Hydatidiform Mole DNA Methylation medicine.disease female genital diseases and pregnancy complications Trophoblasts 030104 developmental biology medicine.anatomical_structure Endocrinology Cell Transformation Neoplastic Reproductive Medicine embryonic structures Uterine Neoplasms Cancer research Female Developmental Biology |
Zdroj: | Placenta. 39 |
ISSN: | 1532-3102 |
Popis: | Introduction Up to 20% of hydatidiform moles are followed by malignant transformation in gestational trophoblastic neoplasia and require chemotherapy. Syncytin-1 is involved in human placental morphogenesis and is also expressed in various cancers. We assessed the predictive value of the expression of Syncytin-1 and its interactants in the malignant transformation process of hydatidiform moles. Methods Syncytin-1 glycoprotein was localized by immunohistochemistry in hydatidiform moles, gestational trophoblastic neoplasia and control placentas. The transcription levels of its locus ERVWE1, its interaction partners (hASCT1, hASCT2, TLR4 and DC-SIGN) and two loci (ERVFRDE1 and ERV3) involved the expression of other placental envelopes were assessed by real-time PCR. Results Syncytin-1 glycoprotein was expressed in syncytiotrophoblast of hydatidiform moles with an apical enhancement when compared with normal placentas. Moles with further malignant transformation had a higher staining intensity of Syncytin-1 surface unit C-terminus but the transcription level of its locus ERVWE1 was not different from that of moles with further remission and normal placentas. hASCT1 and TLR4, showed lower transcription levels in complete moles when compared to normal placentas. ERVWE1, ERVFRDE1 and ERV3 transcription was down-regulated in hydatidiform moles and gestational trophoblastic neoplasia. Conclusions Variations of Syncytin-1 protein localization and down-regulation of hASCT1 and TLR4 transcription are likely to reflect altered functions of Syncytin-1 in the premalignant context of complete moles. The reduced transcription in gestational trophoblastic diseases of ERVWE1, ERVFRDE1 and ERV3, which expression during normal pregnancy is differentially regulated by promoter region methylation, suggest a joint dysregulation mechanism in malignant context. |
Databáze: | OpenAIRE |
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