PSEUDOURIDINE FROM CULTURE MEDIA OF AGROBACTERIUM TUMEFACIENS

Autor: T Suzuki, R M Hochster
Rok vydání: 1964
Předmět:
Zdroj: Canadian Journal of Microbiology. 10:867-875
ISSN: 1480-3275
0008-4166
DOI: 10.1139/m64-113
Popis: Cultures of Agrobacterium tumefaciens grown on a synthetic medium containing glucose plus a pyrimidine produced a pyrimidine riboside with unusual chemical properties. This substance was isolated in crystalline form and identified as 5-ribosyluracil (pseudouridine) on the basis of data obtained from ion exchange and paper chromatography, electrophoresis, ultraviolet and infrared spectrophotometry, color reactions, melting point, elementary analysis, hydrazinolysis, and periodate oxidation followed by bromine treatment.
Databáze: OpenAIRE