Effects of Different Concentrations of Bovine Follicular Fluid and Estrous Cow Serum on Development of Murine 2-Cell Embryos
| Autor: | Parviz Tajik, Hamid Ghasemzadeh-nava, R Beheshti-Govij, J Soleimani-Rad, A Mohamadi-Roshandeh |
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| Rok vydání: | 2008 |
| Předmět: |
Serum
Morula Embryo Culture Techniques Andrology Mice Estrus medicine Animals Humans Blastocyst Bovine serum albumin Cells Cultured Estrous cycle Mice Inbred BALB C biology Chemistry Embryo culture Embryo biology.organism_classification Follicular fluid Follicular Fluid medicine.anatomical_structure Streptomycin embryonic structures biology.protein Cattle Female Agronomy and Crop Science medicine.drug Bovinae |
| Zdroj: | Pakistan Journal of Biological Sciences. 11:628-632 |
| ISSN: | 1028-8880 |
| DOI: | 10.3923/pjbs.2008.628.632 |
| Popis: | Murine 2-cells embryos were isolated from murine oviducts at laboratory and transferred into Ham's F-10 medium containing 0.1 mg mL(-1) streptomycin and 100 IU mL(-1) penicillin G and supplemented with 3 mg mL(-1) bovine serum albumin (BSA) or different concentrations of bovine follicular fluid (bFF) and estrous cow serum (ECS). Significantly higher (p0.05)or =4-cell embryos were developed when embryos were cultured 20% bFF (84.33%) comparing to 10 and 15% bFF (48.33 and 69.33%) as well as 3 mg mL(-1) BSA (65.66%). Morula rates were also lower in 10% bFF (22.33%) comparing to the other groups and were similar in 15 and 20% bFF (62.66 and 72.33% morula rates) as well as BSA containing media (55.33%). The highest (p0.05) blastocyst rates were obtained in medium containing 20% bFF (64.33%) and the lowest belonged to 10% bFF (15%) comparing to 15% bFF (33.66%) or 3 mg mL(-1) BSA. When embryos were cultured in ECS, no significant different was observed in different culture media (76.66, 72.33, 82.5 and 65.66%or =4-cell embryos in 10, 15 and 20% bFF and 3 mg mL(-1) BSA, respectively). Morula and blastocyst rates were also similar in all groups (32.33, 41.66 and 66.25 and 55.33% morula rates and 15.33, 27, 44.50 and 29.66% blastocyst rates for 10, 15 and 20% bFF and 3 mg mL(-1) BSA, respectively). The results of the present study demonstrated that 20% bFF could be substituted for BSA when in vitro culture of murine embryos is carried. |
| Databáze: | OpenAIRE |
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