Quantification of the expression level of the gene encoding the catalytic subunit of telomerase in testicular tissue specimens predicts successful sperm recovery
| Autor: | Mark Schrader, Wolfgang Schulze, Bernd Straub, Kurt Miller, Rüdiger Heicappell, Hans Krause, Markus Müller |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Male endocrine system Telomerase Biopsy Gene Expression Testicle Biology Sensitivity and Specificity Testis Gene expression medicine Humans Telomerase reverse transcriptase RNA Messenger Spermatogenesis Infertility Male Cryopreservation Azoospermia Sertoli Cells medicine.diagnostic_test Reverse Transcriptase Polymerase Chain Reaction Rehabilitation Discriminant Analysis Obstetrics and Gynecology Oligospermia Syndrome medicine.disease Molecular biology Sperm Spermatogonia medicine.anatomical_structure Reproductive Medicine Tissue and Organ Harvesting |
| Zdroj: | Human Reproduction. 17:150-156 |
| ISSN: | 1460-2350 |
| DOI: | 10.1093/humrep/17.1.150 |
| Popis: | BACKGROUND: The objective of the present study was to evaluate the quantitative detection of human telomerase reverse transcriptase (hTERT) mRNA as a new molecular diagnostic parameter in the work-up of testicular tissue specimens from patients presenting with non-obstructive azoospermia. METHODS: hTERT mRNA expression was quantified in 49 cryopreserved testicular tissue specimens by fluorescence real-time RT–PCR in a LightCycler ® . This was paralleled by conventional histological work-up in all tissue specimens and additional semithin sectioning preparation in cases with maturation arrest (n 20) and Sertoli cell-only syndrome (SCOS; n 12). RESULTS: The average normalized hTERT expression (NhTERT) was 136.1 41.7 copies (mean standard deviation) in tissue specimens with presence of haploid germs cells, NhTERT 48.2 21.0 copies in those with maturation arrest and N hTERT 2.7 2.8 copies in those with SCOS. The discriminant analysis showed that detection of N hTERT was able correctly to classify 89.0% of the investigated tissue specimens. CONCLUSIONS: Our results demonstrate that quantitative detection of hTERT mRNA expression in testicular tissue enables a molecular–diagnostic subclassification of spermatogenesis disorders. Quantitative detection of hTERT in testicular biopsies is thus well suited for predicting successful sperm recovery in patients with azoospermia and is a useful molecular diagnostic parameter for supplementing the histopathological evaluation. |
| Databáze: | OpenAIRE |
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