Bio-CaRGOS: capture and release gels for optimized storage of hemoglobin
| Autor: | Chinmay S. Potnis, Kendall Hartline, Theodore S. Kalbfleisch, Rajat Chauhan, Jack Boylan, Gautam Gupta, Robert S. Keynton, Meenakshi Bansal, Kavya Koneru |
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| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Aqueous solution Chromatography Downstream processing Chemistry General Chemical Engineering Refrigeration 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology 03 medical and health sciences Degradation (geology) Current technology Hemoglobin 0210 nano-technology 030304 developmental biology |
| Zdroj: | RSC Advances. 11:13034-13039 |
| ISSN: | 2046-2069 |
| DOI: | 10.1039/d1ra00987g |
| Popis: | Room temperature biospecimen storage for prolonged periods is essential to eliminate energy consumption by ultra-low freezing or refrigeration-based storage techniques. State of the art practices that sufficiently minimize the direct or hidden costs associated with cold-chain logistics include ambient temperature storage of biospecimens (i.e., DNA, RNA, proteins, lipids) in the dry state. However, the biospecimens are still well-exposed to the stress associated with drying and reconstitution cycles, which augments the pre-analytical degradation of biospecimens prior to their downstream processing. An aqueous storage solution that can eliminate these stresses which are correlated to several cycles of drying/rehydration or freezing of biospecimens, is yet to be achieved by any current technology. In our study, we have addressed this room temperature biospecimen-protection challenge using aqueous capture and release gels for optimized storage (Bio-CaRGOS) of biospecimens. Herein, we have demonstrated a single-step ∼95% recovery of a metalloprotein hemoglobin at room temperature using a cost-effective standard microwave-based aqueous formulation of Bio-CaRGOS. Although hemoglobin samples are currently stored at sub-zero or under refrigeration (4 °C) conditions to avoid loss of integrity and an unpredictable diagnosis during their downstream assays, our results have displayed an unprecedented room temperature integrity preservation of hemoglobin. Bio-CaRGOS formulations efficiently preserve hemoglobin in its native state, with single-step protein recovery of ∼95% at ambient conditions (1 month) and ∼96% (7 months) under refrigeration conditions. In contrast, two-thirds of the control samples degrade under ambient (1 month) and refrigeration (7 months) settings. |
| Databáze: | OpenAIRE |
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