Prevascularization of cardiac patch on the omentum improves its therapeutic outcome
| Autor: | Natalie Landa, Radka Holbova, Jonathan Leor, Oren Levy, Shani Dror, Emil Ruvinov, Tal Dvir, Micha S. Feinberg, Inbar Freeman, Yoram Etzion, Alon Kedem, Smadar Cohen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Cardiac function curve
Male medicine.medical_specialty Transplantation Heterotopic medicine.medical_treatment Cell Culture Techniques Myocardial Infarction Scars Neovascularization Physiologic Neovascularization Rats Sprague-Dawley Electrocardiography Tissue engineering Internal medicine medicine Animals Myocardial infarction Cells Cultured Heart transplantation Multidisciplinary medicine.diagnostic_test Tissue Engineering business.industry Graft Survival Cell Differentiation Anatomy Biological Sciences medicine.disease Rats Transplantation Treatment Outcome Cardiology cardiovascular system Microscopy Electron Scanning Heart Transplantation medicine.symptom business Omentum |
| Popis: | The recent progress made in the bioengineering of cardiac patches offers a new therapeutic modality for regenerating the myocardium after myocardial infarction (MI). We present here a strategy for the engineering of a cardiac patch with mature vasculature by heterotopic transplantation onto the omentum. The patch was constructed by seeding neonatal cardiac cells with a mixture of prosurvival and angiogenic factors into an alginate scaffold capable of factor binding and sustained release. After 48 h in culture, the patch was vascularized for 7 days on the omentum, then explanted and transplanted onto infarcted rat hearts, 7 days after MI induction. When evaluated 28 days later, the vascularized cardiac patch showed structural and electrical integration into host myocardium. Moreover, the vascularized patch induced thicker scars, prevented further dilatation of the chamber and ventricular dysfunction. Thus, our study provides evidence that grafting prevascularized cardiac patch into infarct can improve cardiac function after MI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|