A human follicular lymphoma B cell line hypermutates its functional immunoglobulin genesin vitro
| Autor: | Hongyan Wu, Eila Pelkonen, Sakari Knuutila, Matti Kaartinen |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Somatic cell
Molecular Sequence Data Immunology Naive B cell Follicular lymphoma Somatic hypermutation Apoptosis Biology 03 medical and health sciences 0302 clinical medicine Tumor Cells Cultured medicine Humans Immunology and Allergy Amino Acid Sequence Lymphoma Follicular B cell 030304 developmental biology B-Lymphocytes 0303 health sciences Base Sequence Genes Immunoglobulin medicine.disease Molecular biology 3. Good health Raji cell B-1 cell medicine.anatomical_structure Mutation biology.protein Antibody 030215 immunology |
| Zdroj: | European Journal of Immunology. 25:3263-3269 |
| ISSN: | 1521-4141 0014-2980 |
| Popis: | The functional immunoglobulin (Ig) genes of B lymphocytes undergo somatic mutations during immune responses. These mutations modify the antigen binding site of the immunoglobulins, thereby enhancing the average affinity of the antibodies produced. The molecular mechanism underlying these B cell hypermutations remains unresolved, partly because it is difficult to grow normal B cells in long-term cell cultures and because there is no suitable transformed or malignant B cell line which generates mutations in its immunoglobulin genes in vitro. Here, we show that the recently established follicular lymphoma line HF-1.3.4 generates somatic hypermutations in vitro at a high frequency of 0.7 x 10(-6) mutations per base pair per generation in standard cell cultures (RPMI 1640 + 5% fetal calf serum). This shows for the first time that B cell hypermutation can occur without T cells or T cell factors. The mutation frequency increased approximately tenfold to 1 x 10(-5) mutations/base pair/generation with B cell-specific growth factors (interleukins-2 and -4 and three antibodies stimulatory to HF-1.3.4 cells). This HF-1.3.4 lymphoma line may help to elucidate the molecular mechanism of Ig gene hypermutation. |
| Databáze: | OpenAIRE |
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