RNA silencing is resistant to low-temperature in grapevine
| Autor: | Jean E. Masson, Mireille Perrin, Isabelle Soustre-Gacougnolle, Carine Schmitt, Hervé Vaucheret, Taline Elmayan, Jérôme Mutterer, Marjorie Romon, Patrice Dunoyer, Yannick Burdloff, Elodie Chevalier, Aude Zimmermann, Thomas Montavon, Jérôme Zervudacki, Christophe Himber |
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| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, Laboratoire Vigne, Biotechnologies et Environnement, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA)), Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, INRA-GAP-BV (Institut National de la Recherche Agronomique), CIVA (Comite Interprofessionnel des Vins d'Alsace), Conseil Regional Alsace, CIVA-Conseil Regional Alsace, Agence National pour la Recherche [ANR-10-LABX-36], Bettencourt-Schueller foundation |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Small interfering RNA Science [SDV]Life Sciences [q-bio] Green Fluorescent Proteins Arabidopsis Genetically modified crops [SDV.BC]Life Sciences [q-bio]/Cellular Biology 01 natural sciences Virus 03 medical and health sciences RNA interference Gene silencing Arabidopsis thaliana Vitis Transgenes RNA Small Interfering ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences Multidisciplinary biology Inverted Repeat Sequences [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology biology.organism_classification Plants Genetically Modified Cold Temperature RNA silencing Medicine RNA Interference Cell Division 010606 plant biology & botany Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2013, 8 (12), pp.e82652 PLoS ONE, Public Library of Science, 2013, 8 (12), pp.e82652. ⟨10.1371/journal.pone.0082652⟩ PLoS ONE, Vol 8, Iss 12, p e82652 (2013) Plos One 12 (8), e82652. (2013) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0082652⟩ |
| Popis: | RNA silencing is a natural defence mechanism against viruses in plants, and transgenes expressing viral RNA-derived sequences were previously shown to confer silencing-based enhanced resistance against the cognate virus in several species. However, RNA silencing was shown to dysfunction at low temperatures in several species, questioning the relevance of this strategy in perennial plants such as grapevines, which are often exposed to low temperatures during the winter season. Here, we show that inverted-repeat (IR) constructs trigger a highly efficient silencing reaction in all somatic tissues in grapevines. Similarly to other plant species, IR-derived siRNAs trigger production of secondary transitive siRNAs. However, and in sharp contrast to other species tested to date where RNA silencing is hindered at low temperature, this process remained active in grapevine cultivated at 4°C. Consistently, siRNA levels remained steady in grapevines cultivated between 26°C and 4°C, whereas they are severely decreased in Arabidopsis grown at 15°C and almost undetectable at 4°C. Altogether, these results demonstrate that RNA silencing operates in grapevine in a conserved manner but is resistant to far lower temperatures than ever described in other species. |
| Databáze: | OpenAIRE |
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