Anti-biofilm activity of LC-MS based Solanum nigrum essential oils against multi drug resistant biofilm forming P. mirabilis
| Autor: | Sami AlYahya, Khalid F. Alanzi, C. Chenthis Kanisha, Jamal M. Khaled, Shine Kadaikunnan, Natesan Manoharan, Rtv Vimala, Govindan Ramachandran, Govindan Rajivgandhi, Naiyf S. Alharbi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gram-negative bacteria Virulence Solanum nigrum 01 natural sciences law.invention Microbiology 03 medical and health sciences Antibiotic resistance Viability of cells law Liquid chromatography–mass spectrometry Exopolysaccharide lcsh:QH301-705.5 biology Chemistry Biofilm biology.organism_classification 030104 developmental biology Intracellular changes lcsh:Biology (General) Essential oils Multi drug resistant Electron microscope General Agricultural and Biological Sciences 010606 plant biology & botany |
| Zdroj: | Saudi Journal of Biological Sciences, Vol 28, Iss 1, Pp 302-309 (2021) |
| Popis: | Urinary tract infections are second most important diseases worldwide due to the increased amount of antibiotic resistant microbes. Among the Gram negative bacteria, P. mirabilis is the dominant biofilm producer in urinary tract infections next to E. coli. Biofilm is a process that produced self-matrix of more virulence pathogens on colloidal surfaces. Based on the above fact, this study was concentrated to inhibit the P. mirabilis biofilm formation by various in-vitro experiments. In the current study, the anti-biofilm effect of essential oils was recovered from the medicinal plant of Solanum nigrum, and confirmed the available essential oils by liquid chromatography-mass spectroscopy analysis. The excellent anti-microbial activity and minimum biofilm inhibition concentration of the essential oils against P. mirabilis was indicated at 200 µg/mL. The absence of viability and altered exopolysaccharide structure of treated cells were showed by biofilm metabolic assay and phenol–sulphuric acid method. The fluorescence differentiation of P. mirabilis treated cells was showed with more damages by confocal laser scanning electron microscope. Further, more morphological changes of essential oils treated cells were differentiated from normal cells by scanning electron microscope. Altogether, the results were reported that the S. nigrum essential oils have anti-biofilm ability. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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