Type II phosphatidylinositol 4-kinase beta is an integral signaling component of early T cell activation mechanisms
| Autor: | Aparna Ratheesh, Naveen Bojjireddy, Shubhada V. Chiplunkar, Dakshayini P. Kulkarni, Gosukonda Subrahmanyam, Ranjeet Kumar Sinha, Rajiv P. Gude |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Kinase
CD3 Complex T cell T-Lymphocytes Biology Lymphocyte Activation Biochemistry Jurkat cells chemistry.chemical_compound Jurkat Cells Transduction medicine Cell Adhesion Golgi Humans Splenic Lymphocytes Phosphatidylinositol Calcium Signaling Phosphorylation Receptor Cell adhesion 1-Phosphatidylinositol 4-Kinase Calcium signaling Microscopy Confocal Membrane Receptor-Zeta-Chain Antigen Receptor General Medicine Actin cytoskeleton Cell biology Actin Cytoskeleton medicine.anatomical_structure chemistry Signal transduction Lipid Kinases Organization T Cell Activation |
| Zdroj: | IndraStra Global. |
| ISSN: | 2381-3652 |
| Popis: | The early signaling events in T cell activation through CD3 receptor include a rapid change in intra cellular free calcium concentration and reorganization of actin cytoskeleton. Phosphatidylinositol 4-kinases (Ptdlns 4-kinases) are implicated as key components in these early signaling events. The role of type II PtdIns 4-kinase beta in CD3 receptor signaling was investigated with the help of short hairpin RNA sequences. Cross-linking of CD3 receptors on Jurkat T Cells with monoclonal antibodies showed an early increase in type II PtdIns 4-kinase activity and co-localization of type II PtdIns 4-kinase beta with CD3 zeta. Transfection of Jurkat T Cells with shRNAs inhibited CD3 receptor mediated type II PtdIns 4-kinase activation with a concomitant reduction in intra cellular calcium release, suggesting a role for type II PtdIns 4-kinase beta in CD3 receptor signal transduction. Knock-down of type II PtdIns 4-kinase beta with shRNAs also correlated with a decrease in PtdIns 4-kinase activity in cytoskeleton fractions and reduced adhesion to matrigel surfaces. These results indicate that type II Ptdlns 4-kinase beta is a key component in early T cell activation signaling cascades. Published by Elsevier Masson SAS. |
| Databáze: | OpenAIRE |
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