Expression of OX40 Gene and its Serum Levels in Neuromyelitis Optica Patients
| Autor: | Nahid Eskandari, Arezou Dabiri, Parya Alidadiani, Vahid Shaygannejad, Hamidreza Jahanbani-Ardakani, Omid Mirmosayyeb, Mostafa Manian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male QH301-705.5 medicine.medical_treatment Central nervous system General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Immune system medicine Humans CD134 RNA Messenger Biology (General) Autoimmune disease Neuromyelitis optica biology business.industry Neuromyelitis Optica ox40 autoimmune General Medicine Receptors OX40 medicine.disease 030104 developmental biology Cytokine medicine.anatomical_structure Case-Control Studies Immunology biology.protein Tumor necrosis factor alpha Female Antibody business 030217 neurology & neurosurgery neuromyelitis optica (nmo) |
| Zdroj: | Biomolecular Concepts, Vol 10, Iss 1, Pp 62-67 (2019) |
| ISSN: | 1868-5021 |
| Popis: | Neuromyelitis optica (NMO), also known as Devic’s disease, is an autoimmune disorder of the central nervous system (CNS) in which immune system cells and antibodies primarily attack the optic nerves and the spinal cord. OX40 (CD134) is a tumor necrosis factor (TNF)-receptor family member expressed primarily on activated CD4+ and CD8+ T-cells. In an autoimmune disease, OX40 is typically up-regulated at sites of inflammation, and increases in the number of peripheral CD4+ T-cells expressing OX40. OX40 and its ligand OX40L are key TNF members that augment T-cell expansion, cytokine production, and promote T-cell survival. The aim of this study was to evaluate and compare of OX40 gene expression and its serum levels in patients with NMO and healthy controls. Twenty sex-/age-matched healthy controls (HC) (median age = 32 years, 15 females/5 males) were engaged for the present study. Expression of OX40 at the transcript level and serum protein levels were measured by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assays, respectively. The results indicated OX40 expression in patients was significantly lower than in healthy controls (p = 0.001). However, the serum level of OX40 was not significantly different between groups (p = 0.37). In addition, the results indicated that CD134 expression was not age-related (p = 0.041). Overall, this study suggests to us that OX40 levels are not a suitable marker for diagnosis or treatment of NMO. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|