Flow cytometry evaluation of CD14/CD16 monocyte subpopulations in systemic sclerosis patients: a cross sectional controlled study
| Autor: | Rafael Mendonça da Silva Chakr, Carolina Yuka Ueda, Isadora Flesch da Silva Moreira, Ricardo Machado Xavier, Vanessa Hax, Rafaella Romeiro Piovesan, Laiana Schneider, Natália Aydos Marcondes |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Monocitos medicine.medical_specialty CD14 Lipopolysaccharide Receptors Diseases of the musculoskeletal system Pathogenesis GPI-Linked Proteins Monocytes 03 medical and health sciences 0302 clinical medicine Rheumatology Fibrosis Internal medicine medicine Humans Flow cytometry skin and connective tissue diseases 030203 arthritis & rheumatology Autoimmune disease CD64 Scleroderma Systemic integumentary system business.industry Monocyte Receptors IgG HLA-DR Antigens RC581-607 Escleroderma sistêmico Flow Cytometry medicine.disease Monocyte subpopulations Cross-Sectional Studies 030104 developmental biology medicine.anatomical_structure RC925-935 Immunology Systemic sclerosis Immunologic diseases. Allergy business CD163 Citometria de fluxo |
| Zdroj: | Advances in Rheumatology, Volume: 61, Article number: 27, Published: 04 JUN 2021 Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS Advances in Rheumatology, Vol 61, Iss 1, Pp 1-11 (2021) |
| ISSN: | 2523-3106 |
| DOI: | 10.1186/s42358-021-00182-8 |
| Popis: | Background Systemic sclerosis (SSc) is a chronic autoimmune disease characterized by vasculopathy and fibrosis, which can be subclassified into diffuse cutaneous (dSSc) and limited cutaneous (lSSc) subtypes. Previous studies suggest that an increase in monocytes can be a hallmark of various inflammatory diseases, including SSc. Our aim was to evaluate circulating blood monocyte subpopulations (classical, intermediate and non-classical) of SSc patients and their possible association with disease manifestations. Methods Fifty consecutive patients fulfilling the 2013 ACR/EULAR classification criteria for SSc were included in a cross-sectional study. Monocyte subpopulations were identified based on their expression of CD64, CD14 and CD16, evaluated by flow cytometry, and were correlated with the clinical characteristics of the patients; furthermore, the expression of HLA-DR, CD163, CD169 and CD206 in the monocytes was studied. Thirty-eight age- and sex-matched healthy individuals were recruited as a control group. Results SSc patients had an increased number of circulating peripheral blood monocytes with an activated phenotypic profile compared to healthy subjects. Absolute counts of CD16+ (intermediary and non-classical) monocyte subpopulations were higher in SSc patients. There was no association between monocyte subpopulations and the clinical manifestations evaluated. Conclusion We identified higher counts of all monocyte subpopulations in SSc patients compared to the control group. There was no association between monocyte subpopulations and major fibrotic manifestations. CD169 was shown to be more representative in dSSc, being a promising marker for differentiating disease subtypes. |
| Databáze: | OpenAIRE |
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