Iloprost for the attenuation of ischaemia/reperfusion injury in a distant organ
| Autor: | Murat Dikmengil, Ayse Polat, Ulas Degirmenci, Nehir Sucu, Lülüfer Tamer, Ali Gül, Ilhan Mavioglu, Barlas Aytacoglu |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Sodium medicine.medical_treatment Clinical Biochemistry Ischemia chemistry.chemical_element Protective Agents Biochemistry chemistry.chemical_compound Internal medicine Malondialdehyde medicine Animals Iloprost Rats Wistar Saline Peroxidase Adenosine Triphosphatases Kidney biology business.industry Cell Biology General Medicine medicine.disease Hindlimb Rats Endocrinology medicine.anatomical_structure chemistry Myeloperoxidase Anesthesia Reperfusion Injury biology.protein Kidney Diseases business Reperfusion injury medicine.drug |
| Zdroj: | Cell biochemistry and function. 24(4) |
| ISSN: | 0263-6484 |
| Popis: | The objective of this study was to investigate antioxidant and cytoprotective properties of iloprost in a distant organ after ischaemia reperfusion injury. Male Wistar rats were divided into two groups. After application of anesthaesia both hindlimbs were occluded. A 2-h reperfusion procedure was carried out after 60 min of ischemia. Study group (STU) rats (n=10) received 10 microg kg(-1) iloprost in 1 ml of saline from the tail vein 10 min before reperfusion. Control (CON) group rats (n=10) received an equal amount of saline. The rats were sacrificed by injection of a high dose of thiopentone sodium. Blood and tissue samples (right kidneys) were taken for analysis. Differences in malondialdehyde (MDA), myeloperoxidase (MPO), Na+-K+ ATPase and total antioxidant capacity (TAC) between the groups were analysed. MPO, MDA and TAC levels in the sera of CON and STU groups were 1.60+/-0.26 U l(-1), 11.42+/-5.23 nmol ml(-1), 8.30 x 10(-2)+/- 3.93 x 10(-2) nmol ml(-1) h(-1) and 1.07+/-0.11 U l(-1), 7.60+/-1.81 nmol ml(-1) and 0.15+/-3.23 x 10(-2) nmol ml(-1) h(-1) (p=0.0001, p=0.043 and p=0.0001 respectively). MPO, ATPase and MDA levels in kidneys for CON and STU groups were 1.24+/-0.58 U g(-1), 85.70+/-52.05 nmol mg(-1), 17.90+/-7.40 nmol ml(-1) and 0.78+/-0.31 U g(-1), 195.90+/-56.13 nmol mg(-1) and 10.10+/-0.99 nmol ml(-1) (p=0.046, p=0.0001 and p=0.009 respectively). When given prior to reperfusion, the positive effect of iloprost in the attenuation of distant organ reperfusion injury has been demonstrated. |
| Databáze: | OpenAIRE |
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