A Rapid Procedure Suitable to Assess Quantitatively the Endocytosis of Colloidal Gold and Its Conjugates in Cultured Cells
| Autor: | J J Martin de Llano, Erwin Knecht, I Moreno, Enrique J. Andreu |
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| Rok vydání: | 1998 |
| Předmět: |
0301 basic medicine
Time Factors Histology Endocytic cycle CHO Cells Gold Colloid Endocytosis Mass spectrometry Mass Spectrometry 03 medical and health sciences Cricetinae Animals Inductively coupled plasma mass spectrometry Cells Cultured Chromatography 030102 biochemistry & molecular biology Histocytochemistry Chemistry Lipoproteins LDL Microscopy Electron 030104 developmental biology Evaluation Studies as Topic Colloidal gold Anatomy Inductively coupled plasma |
| Zdroj: | ResearcherID |
| ISSN: | 1551-5044 0022-1554 |
| Popis: | We measured the endocytic uptake of low-density lipoproteins (LDLs) conjugated to colloidal gold in cultured cells, either by counting gold particles on electron micrographs or by inductively coupled plasma (ICP) mass spectrometry (MS). Both procedures are comparable but the latter requires a considerably shorter time and allows analysis of a much larger sample. In addition, ICP MS, compared to alternative radioactive or fluorescent procedures, offers the major advantage of using the same probe to quantify the endocytic uptake and to follow it by electron microscopy. Therefore, ICP MS analysis provides an easy, rapid, and sensitive quantification of endocytosis that complements the electron microscopic studies. |
| Databáze: | OpenAIRE |
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