The expression of APRIL in Sjogren's syndrome: aberrant expression of APRIL in the salivary gland
| Autor: | Gabor G. Illei, Eline J. Polling, Paul Peter Tak, Nienke Roescher, Jelle L. Vosters |
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| Přispěvatelé: | Clinical Immunology and Rheumatology, Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Saliva Transmembrane Activator and CAML Interactor Protein medicine.medical_treatment Tumor Necrosis Factor Ligand Superfamily Member 13 Salivary Glands Minor Pathogenesis Basic Science Rheumatology stomatognathic system Internal medicine B-Cell Activating Factor Biopsy Image Processing Computer-Assisted Humans Medicine Pharmacology (medical) B-cell activating factor Salivary gland medicine.diagnostic_test business.industry Transmembrane activator and CAML interactor Sjogren's Syndrome Cytokine Endocrinology medicine.anatomical_structure Immunology Immunohistochemistry business Biomarkers |
| Zdroj: | Rheumatology (Oxford, England), 51(9), 1557-1562. Oxford University Press |
| ISSN: | 1462-0324 |
| DOI: | 10.1093/rheumatology/kes080 |
| Popis: | Objective. A proliferation-inducing ligand (APRIL) and B-cell activating factor (BAFF) are B-cell-related mediators and may play a role in the pathogenesis in SS. In this descriptive study we assessed the expression of APRIL and BAFF in the minor salivary gland and serum from SS patients. Methods. Paraffin-embedded minor salivary gland sections from SS patients, non-SS controls and healthy volunteers were analysed by immunohistochemistry. Digital image quantification was performed to evaluate the expression of BAFF, APRIL and transmembrane activator and CAML interactor. Furthermore, serum was analysed for soluble BAFF and APRIL levels by ELISA. All the data were also analysed for subjects with decreased and normal stimulated salivary flow independent of the classification. Results. APRIL expression was lower in minor salivary gland biopsies from SS patients compared with healthy volunteers and to a lesser extent non-SS controls, whereas BAFF expression was similar in all groups. Soluble APRIL levels in serum were increased in SS patients and in subjects with decreased salivary flow independent of the classification. Conclusion. APRIL salivary gland tissue levels are decreased, suggesting that targeting this cytokine locally in the salivary glands would not benefit SS patients. Moreover, the discrepancy between local and systemic levels is striking and future research should assess this in more detail |
| Databáze: | OpenAIRE |
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