Expression of MRP14 gene is frequently down-regulated in Chinese human esophageal cancer
| Autor: | Bao Sheng Chen, Ming Rong Wang, Zhi Xiong Xu, Xin Xu, Yan Cai, Ya Ling Han, Hao Xu, Jie Wang, Hai Hu, Jun Zhao, Min Wu |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Male China DNA Complementary Esophageal Neoplasms Blotting Western DNA Mutational Analysis Down-Regulation Adenocarcinoma Biology Epithelium Cell Line Tumor Complementary DNA medicine Calgranulin B Humans RNA Neoplasm Northern blot Esophagus Molecular Biology Aged Neoplasm Staging Cell Nucleus Regulation of gene expression Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Cell Biology Middle Aged Esophageal cancer Blotting Northern medicine.disease Immunohistochemistry Molecular biology Gene Expression Regulation Neoplastic Gene expression profiling Blot medicine.anatomical_structure Carcinoma Squamous Cell ATP-Binding Cassette Transporters Female |
| Zdroj: | Cell Research. 14:46-53 |
| ISSN: | 1748-7838 1001-0602 |
| DOI: | 10.1038/sj.cr.7290201 |
| Popis: | Migration inhibitory factor-related protein 14 (MRP14) is one of calcium-binding proteins, referred as S100A9. The heterodimeric molecule formed by MRP14 with its partner MRP8 (S100A8) is the major fatty acid carrier in neutrophils. The MRP8/14 complex has been also implicated in the intracellular transport of arachidonic acid and its precursors in keratinocytes. We show here the involvement of MRP14 in human esophageal cancer. In an initial study, mRNA differential display-reverse transcription polymerase chain reaction (DD-PCR) was performed with two esophageal carcinomas, one esophageal adenocarcinoma and matched normal adjacent mucosa. DD-PCR with the arbitrary primer OPA3 showed that one cDNA band was highly expressed in normal tissues, but disappeared or substantially decreased in tumor counterparts. It was later identified to be the 3'-end of migration inhibitory factor-related protein 14 (MRP14). Northern blotting, RT-PCR and Western blotting corroborated the down-regulation of MRP14 in 58/64 squamous cell carcinomas and 2/2 adenocarcinomas as compared with adjacent normal epithelia of the esophagus. MRP14 was undetectable in 3/3 esophageal-carcinoma cell lines. Immunochemistry demonstrated that expression of MRP14 was restricted to normal esophageal epithelia. No mutation was found in the genomic DNA of the MRP14 gene by PCR and directed DNA sequencing. Our finding suggested that the reduction of MRP14 expression is a frequent event in Chinese human esophageal cancer. |
| Databáze: | OpenAIRE |
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