Enumeration of Rare Cells in Whole Blood by Signal Ion Emission Reactive Release Amplification with Same-Sample RNA Analysis
| Autor: | Cao Zehui, Frédérique Deiss, Michael Pugia, M. Regina Barron, Anna Vorsilak, Zane Baird |
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| Rok vydání: | 2019 |
| Předmět: |
Microfluidics
Cell Count 010402 general chemistry Real-Time Polymerase Chain Reaction 01 natural sciences Signal Mass Spectrometry Analytical Chemistry law.invention law Cell Line Tumor Enumeration Leukocytes Humans Nanotechnology Fluidics Multiplex RNA Messenger Filtration Detection limit Ions Chromatography Chemistry 010401 analytical chemistry Optical Imaging Microfluidic Analytical Techniques Sample (graphics) 0104 chemical sciences Chromatography Gel Immunologic Techniques Nanoparticles |
| Zdroj: | Analytical chemistry. 91(3) |
| ISSN: | 1520-6882 |
| Popis: | Herein is presented a platform capable of detecting less than 30 cells from a whole blood sample by size-exclusion filtration, microfluidic sample handling, and mass spectrometric detection through signal ion emission reactive release amplification (SIERRA). This represents an approximate 10-fold improvement in detection limits from previous work. Detection by SIERRA is accomplished through the use of novel nanoparticle reagents coupled with custom fluidic fixtures for precise sample transfer. Sample processing is performed in standardized 96-well microtiter plates with commonly available laboratory instrumentation to facilitate assay automation. The detection system is easily amenable to multiplex detection, and compatibility with PCR-based gene assays is demonstrated. |
| Databáze: | OpenAIRE |
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