Molecular mechanisms of apoptosis induced by magnolol in colon and liver cancer cells
Autor: | Yuan Soon Ho, Yi Hsuan Lee, Wen Sen Lee, Jean Dean Liu, Shyr Yi Lin, Yu Tza Chang, Chung Hsun Yu |
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Rok vydání: | 2001 |
Předmět: |
Cancer Research
Apoptosis Cytochrome c Group Caspase 3 Caspase 8 Lignans chemistry.chemical_compound Tumor Cells Cultured Humans fas Receptor Molecular Biology Caspase biology Cytochrome c Biphenyl Compounds Liver Neoplasms Fibroblasts Antineoplastic Agents Phytogenic Molecular biology Magnolol Cell biology Hep G2 chemistry Caspases Colonic Neoplasms biology.protein Calcium Endothelium Vascular Intracellular Signal Transduction |
Zdroj: | Molecular Carcinogenesis. 32:73-83 |
ISSN: | 1098-2744 0899-1987 |
Popis: | Magnolol has been reported to have anticancer activity. In this study we found that treatment with 100 microm magnolol induced apoptosis in cultured human hepatoma (Hep G2) and colon cancer (COLO 205) cell lines but not in human untransformed gingival fibroblasts and human umbilical vein endothelial cells. Our investigation of apoptosis in Hep G2 cells showed a sequence of associated intracellular events that included (a) increased cytosolic free Ca(2+); (b) increased translocation of cytochrome c (Cyto c) from mitochondria to cytosol; (c) activation of caspase 3, caspase 8, and caspase 9; and (d) downregulation of bcl-2 protein. Pretreatment of the cells with the phospholipase C inhibitor 1-[6-[[(17 beta)-3-methoxyestra-1,3,5(10)-trien-17-yl]amino]hexyl]-1 H-pyrrole-2,5-dione (U73122) or the intracellular chelator of Ca(2+) 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA/AM) inhibited the subsequent magnolol augmentation of [Ca(2+)](i) and also the activation of caspase-8 and caspase-9, so that the occurrence of apoptosis in those cells was greatly reduced. Pretreatment of the cells with ZB4 (which disrupts the Fas response mechanism) also decreased the subsequent magnolol-induced caspase-8 activation and reduced the occurrence of apoptosis. We interpreted these findings to indicate that the above-listed sequence of intracellular events led to the apoptosis seen in Hep G2 cells and that [Ca(2+)](i), Cyto c, and Fas function as intracellular signals to coordinate those events. |
Databáze: | OpenAIRE |
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