Production and purification of Clostridium perfringens type D epsilon toxin and IgY antitoxin
| Autor: | Luiz Guilherme Dias Heneine, Ronnie Antunes de Assis, Luciana Aramuni Gonçalves, Rodrigo Otávio Silveira Silva, Guilherme Guerra Alves, Carlos Augusto Oliveira Junior, Francisco Carlos Faria Lobato |
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| Rok vydání: | 2020 |
| Předmět: |
Clostridium perfringens
Ion chromatography Bacterial Toxins Immunoglobulins medicine.disease_cause Microbiology Enterotoxemia 03 medical and health sciences medicine Potency Animals 030304 developmental biology 0303 health sciences biology 030306 microbiology Chemistry Toxin Antibody titer Antibodies Bacterial Infectious Diseases biology.protein Immunoglobulin Y Antitoxin Chickens |
| Zdroj: | Anaerobe. 69 |
| ISSN: | 1095-8274 |
| Popis: | The aim of this study was to purify Clostridium perfringens type D epsilon toxin and produce and purify anti-epsilon chicken immunoglobulin Y (IgY). A single-step ion exchange chromatography resulted in a high-yield and high-purity toxin, while ion exchange chromatography followed by gel filtration resulted in the highest purity of the toxin, but at a lower yield. Purified and inactivated epsilon toxin were then administered in chickens via four inoculations and IgY was obtained at a high purity and yield, with an antibody titer of 50 IU/mL and high levels of avidity (73.2%). In summary, C. perfringens type D epsilon toxin and chicken anti-epsilon IgY were successfully produced and purified, and may be used for the diagnosis of enterotoxemia caused by the epsilon toxin, as well as in potency tests of existing and future vaccines against enterotoxemia. |
| Databáze: | OpenAIRE |
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