Palmitoylation of CD36/FAT regulates the rate of its post-transcriptional processing in the endoplasmic reticulum
| Autor: | Gordon F. Burns, Rick F. Thorne, Charles E. de Bock, Nizar M. Mhaidat, Andrew W. Boyd, Kylie J. Ralston, Xudong Zhang |
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| Rok vydání: | 2010 |
| Předmět: |
CD36 Antigens
CD36 Lipoylation Acylation Immunoblotting Golgi Apparatus Biology Endoplasmic Reticulum chemistry.chemical_compound Membrane Microdomains Palmitoylation Oxidised low density lipoprotein (oxLDL) Cell Line Tumor Chlorocebus aethiops Animals Humans Endoplasmic reticulum (ER) Cysteine Scavenger receptor Gap-43 protein RNA Processing Post-Transcriptional Lipid raft Molecular Biology Secretory pathway Alanine Endoplasmic reticulum Lipids rafts Cell Membrane Cell Biology Flow Cytometry Cell biology Cerulenin Lipoproteins LDL Protein Transport Biochemistry chemistry Microscopy Fluorescence Detergent-resistant membranes (DRMs) COS Cells Mutation biology.protein lipids (amino acids peptides and proteins) Protein Processing Post-Translational |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1803(11):1298-1307 |
| ISSN: | 0167-4889 |
| DOI: | 10.1016/j.bbamcr.2010.07.002 |
| Popis: | CD36/FAT is a transmembrane glycoprotein that functions in the cellular uptake of long-chain fatty acids and also as a scavenger receptor. As such it plays an important role in lipid homeostasis and, pathophysiologically, in the progression of type 2 diabetes and atherosclerosis. CD36 expression is tightly regulated at the levels of both transcription and translation. Here we show that its expression and location are also regulated post-translationally, by palmitoylation. Although palmitoylation of CD36 was not required for receptor maturation and cell surface expression, inhibition of palmitoylation either pharmacologically with cerulenin or by mutation of the relevant cysteines delayed processing at the ER and trafficking through the secretory pathway. The absence of palmitoylation also reduced the half life of the CD36 protein. Additionally, the CD36 palmitoylation mutant did not incorporate efficiently into lipid rafts, a site known to be required for its function of fatty acid uptake, and this reduced the efficiency of uptake of oxidized low density lipoprotein. These findings provide an added level of sophistication where translocation of CD36 to the plasma membrane may be physiologically regulated by palmitoylation. |
| Databáze: | OpenAIRE |
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