Facile Cholesterol Loading with a New Probe ezFlux Allows for Streamlined Cholesterol Efflux Assays

Autor:	Andrei Maiseyeu, Marcus A. Badgeley, Anastasia M Ravodina, Sanjay Rajagopalan, Daria V Fedyukina
Rok vydání:	2020
Předmět:	Chemistry Cholesterol General Chemical Engineering General Chemistry Article Cell labeling chemistry.chemical_compound Förster resonance energy transfer Biochemistry Efflux QD1-999 Intracellular Lipoprotein
Zdroj:	ACS Omega, Vol 5, Iss 36, Pp 23289-23298 (2020) ACS Omega
ISSN:	2470-1343
DOI:	10.1021/acsomega.0c03112
Popis:	Here, we report a nanoparticle-based probe that affords facile cell labeling with cholesterol in cholesterol efflux (CE) assays. This probe, called ezFlux, was optimized through a screening of multiple nanoformulations engineered with a Förster resonance energy transfer (FRET) reporter. The physicochemical- and bio-similarity of ezFlux to standard semi-synthetic acetylated low-density lipoprotein (acLDL) was confirmed by testing uptake in macrophages, the intracellular route of degradation, and performance in CE assays. A single-step fast self-assembly fabrication makes ezFlux an attractive alternative to acLDL. We also show that CE testing using ezFlux is significantly cheaper than that performed using commercial kits or acLDL. Additionally, we analyze clinical trials that measure CE and show that ezFlux has a place in many research and clinical laboratories worldwide that use CE to assess cellular and lipoprotein function.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d778f9cecef81243af20abbad5c0348b https://doi.org/10.1021/acsomega.0c03112 Zobrazit plný text záznamu