Cloning and expression of a member of the Aspergillus niger gene family encoding α-galactosidase
| Autor: | Peter J. Punt, Ana M. Mateo Rosell, Irma F. den Herder, Caren M. van Zuilen, Cees A. M. J. J. van den Hondel |
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| Rok vydání: | 1992 |
| Předmět: |
Genes
Fungal Molecular Sequence Data Gene Expression HSPA2 Genetics Gene family Genomic library Amino Acid Sequence Cloning Molecular Molecular Biology Gene Peptide sequence Gene Library Base Sequence biology Oligonucleotide Aspergillus niger Nucleic acid sequence biology.organism_classification Molecular biology Blotting Southern Biochemistry Multigene Family alpha-Galactosidase Oligonucleotide Probes Plasmids |
| Zdroj: | Molecular and General Genetics MGG. 233:404-410 |
| ISSN: | 1432-1874 0026-8925 |
| DOI: | 10.1007/bf00265437 |
| Popis: | An enzyme with alpha-galactosidase activity and an apparent molecular weight of 82 kDa was purified from culture medium of Aspergillus niger. The N-terminal amino acid sequence of the purified protein shows similarity to the N-terminal amino acid sequence of alpha-galactosidases from several other organisms. Oligonucleotides, based on the N-terminal amino acid sequence, were used as probes to clone the corresponding gene from a lambda EMBL3 gene library of A. niger. The cloned gene (aglA) was shown to be functional by demonstrating that the 82 kDa alpha-galactosidase is absent from a strain with a disruption of the aglA gene, and is over-produced in strains containing multiple copies of the aglA gene. Enzyme activity assays revealed that the 82 kDa alpha-galactosidase A represents a minor extracellular alpha-galactosidase activity in A. niger. |
| Databáze: | OpenAIRE |
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