Effects of exogenous estrogen on uterine leukocyte recruitment
| Autor: | Carolyn J. Kubik, Julie A. DeLoia, Jill Brekosky, Ann M. Stewart-Akers |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
medicine.medical_specialty Chemokine medicine.drug_class Biopsy medicine.medical_treatment Population Uterus Endometrium Internal medicine Leukocytes medicine Humans Prospective Studies RNA Messenger Interleukin 8 education education.field_of_study biology Reverse Transcriptase Polymerase Chain Reaction Obstetrics and Gynecology Estrogens Middle Aged Immunohistochemistry Chemotaxis Leukocyte medicine.anatomical_structure Endocrinology Cytokine Reproductive Medicine Estrogen In utero biology.protein Female Chemokines |
| Zdroj: | Fertility and Sterility. 77:548-554 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/s0015-0282(01)03062-x |
| Popis: | Objective: To determine the role of estrogen in leukocyte recruitment to the human endometrium. Design: Prospective, controlled in vivo study. Setting: Academic research laboratory. Patient(s): Ten patients presenting for donor oocytes. Intervention(s): Endometrial biopsies for the evaluation of leukocyte populations were collected from perimenopausal women in two consecutive regulated cycles who were given two different regimens of estrogen with identical progesterone treatment. Main Outcome Measure(s): Immunohistochemical identification of endometrial leukocyte populations and relative levels of expression of three chemokine genes. Result(s): The total uterine leukocyte population increased significantly when the women received oral estrogen, which resulted in higher serum estrogen levels. This rise in leukocytes was due to a significant increase in both the uterine natural killer cells and the macrophage populations. T-cell numbers did not change relative to circulating estrogen levels. The relative abundance of mRNA from three chemokines was also determined. No changes were found in the expression of M-CSF or MCP-1. Interleukin 8 decreased in glands relative to estrogen levels. Conclusion(s): These data demonstrate that changes in circulating levels of estrogen can regulate the recruitment of bone marrow-derived cells to the uterine endometrium; however, the mechanism whereby that occurs remains elusive. |
| Databáze: | OpenAIRE |
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