Toll-like receptor 3 promotes cross-priming to virus-infected cells
| Autor: | Peter Liljeström, Margaret Chen, Oliver Schulz, Caetano Reis e Sousa, Richard A. Flavell, Yasu-Taka Azuma, Martijn A. Nolte, Tanja I. Näslund, Sandra S. Diebold, Lena Alexopoulou |
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| Přispěvatelé: | Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Landsteiner Laboratory, Experimental Immunology, Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
viruses
MESH: Membrane Glycoproteins Mice 0302 clinical medicine Chlorocebus aethiops Cytotoxic T cell MESH: Animals Encephalomyocarditis virus MESH: Receptors Cell Surface 0303 health sciences Antigen Presentation MESH: Encephalomyocarditis virus Multidisciplinary Membrane Glycoproteins biology MESH: Dendritic Cells Toll-Like Receptors MESH: Toll-Like Receptor 3 3. Good health Cell biology medicine.anatomical_structure Interleukin 12 [SDV.IMM]Life Sciences [q-bio]/Immunology MESH: Toll-Like Receptors MESH: Cross-Priming Ovalbumin T cell Antigen presentation MESH: Vero Cells Receptors Cell Surface chemical and pharmacologic phenomena Endosomes MESH: Poly I-C 03 medical and health sciences Cross-Priming MESH: RNA Double-Stranded MESH: Mice Inbred C57BL MESH: Cardiovirus Infections medicine Cardiovirus Infections Animals RNA Messenger Antigen-presenting cell Vero Cells MESH: Mice 030304 developmental biology RNA Double-Stranded MESH: RNA Messenger CD40 MESH: Ovalbumin Follicular dendritic cells Dendritic cell Dendritic Cells MESH: Cercopithecus aethiops Toll-Like Receptor 3 Mice Inbred C57BL Poly I-C MESH: Endosomes MESH: Antigen Presentation biology.protein MESH: T-Lymphocytes Cytotoxic 030215 immunology T-Lymphocytes Cytotoxic |
| Zdroj: | Nature Nature, Nature Publishing Group, 2005, 433 (7028), pp.887-92. ⟨10.1038/nature03326⟩ Nature, 433(7028), 887-892. Nature Publishing Group Nature, 2005, 433 (7028), pp.887-92. ⟨10.1038/nature03326⟩ |
| ISSN: | 0028-0836 1476-4679 1476-4687 |
| DOI: | 10.1038/nature03326⟩ |
| Popis: | Cross-presentation of cell-associated antigens plays an important role in regulating CD8+ T cell responses to proteins that are not expressed by antigen-presenting cells (APCs). Dendritic cells are the principal cross-presenting APCs in vivo and much progress has been made in elucidating the pathways that allow dendritic cells to capture and process cellular material. However, little is known about the signals that determine whether such presentation ultimately results in a cytotoxic T cell (CTL) response (cross-priming) or in CD8+ T cell inactivation (cross-tolerance). Here we describe a mechanism that promotes cross-priming during viral infections. We show that murine CD8alpha+ dendritic cells are activated by double-stranded (ds)RNA present in virally infected cells but absent from uninfected cells. Dendritic cell activation requires phagocytosis of infected material, followed by signalling through the dsRNA receptor, toll-like receptor 3 (TLR3). Immunization with virus-infected cells or cells containing synthetic dsRNA leads to a striking increase in CTL cross-priming against cell-associated antigens, which is largely dependent on TLR3 expression by antigen-presenting cells. Thus, TLR3 may have evolved to permit cross-priming of CTLs against viruses that do not directly infect dendritic cells. |
| Databáze: | OpenAIRE |
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