Renal organic anion secretion: evidence for dopaminergic and adrenergic regulation
| Autor: | Patricia A. Halpin, J. L. Renfro |
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| Rok vydání: | 1996 |
| Předmět: |
Anions
medicine.medical_specialty Thapsigargin Physiology Dopamine Oxymetazoline Flounder Calcium in biology Kidney Tubules Proximal chemistry.chemical_compound Adrenergic Agents Physiology (medical) Internal medicine medicine Staurosporine Animals Secretion Kidney Forskolin Probenecid Intracellular Membranes Enzyme Activation medicine.anatomical_structure Endocrinology chemistry Phorbol Calcium 2 4-Dichlorophenoxyacetic Acid Adrenergic alpha-Agonists Protein Kinases medicine.drug |
| Zdroj: | The American journal of physiology. 271(5 Pt 2) |
| ISSN: | 0002-9513 |
| Popis: | To examine possible regulatory control of renal proximal tubule organic anion secretion, winter flounder (Pleuronectes americanus) proximal tubule primary cultures were mounted in Ussing chambers. Unidirectional fluxes of [2,4-(14)C]dichlorophenoxyacetic acid were determined under short-circuited conditions. Phorbol 12-myristate 13-acetate (1 microM) caused a significant (P < 0.01) inhibition of net 2,4-dichlorophenoxyacetic acid secretion. Preincubation with staurosporine (1 microM) blocked the phorbol 12-myristate 13-acetate-induced decrease in secretion. Neither forskolin (10 microM) nor W-7 (20 microM) had any effect on net transport. Elevation of intracellular calcium activity with either A-23187 or thapsigargin produced a slight, transient decrease in transport. Addition of dopamine (1 microM) to the peritubular side, but not the luminal side, caused a significant (P < 0.01) decrease in net secretion. Both the alpha-adrenergic agonist oxymetazoline (10 microM) and depletion of intracellular Na+ transiently, but significantly (P < 0.05), increased net transport. The data indicate that renal organic anion excretion may be regulated through dopaminergic inhibition and alpha-adrenergic stimulation of net transepithelial secretion. |
| Databáze: | OpenAIRE |
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