Schinus terebinthifolius countercurrent chromatography (Part III): Method transfer from small countercurrent chromatography column to preparative centrifugal partition chromatography ones as a part of method development
| Autor: | Fernanda das Neves Costa, Jean-Hugues Renault, Peter Hewitson, Alexis Kotland, Jane Hubert, Svetlana Ignatova, Nicolas Borie |
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| Přispěvatelé: | Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Institut de Chimie Moléculaire de Reims - UMR 7312 (ICMR), SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Brunel University London [Uxbridge] |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Anacardiaceae
Analytical chemistry Ethyl acetate Fractionation Chemical Fractionation 01 natural sciences Biochemistry Chemistry Techniques Analytical Analytical Chemistry chemistry.chemical_compound Schinus terebinthifolius Column chromatography Countercurrent chromatography [CHIM.ANAL]Chemical Sciences/Analytical chemistry Countercurrent Distribution Dichloromethane Heptane Chromatography Plant Extracts 010405 organic chemistry Methanol 010401 analytical chemistry Organic Chemistry Water Centrifugal partition chromatography General Medicine [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences Triterpenes 0104 chemical sciences Radial chromatography chemistry Fruit Solvents Method transfer Chromatography column Chromatography Liquid |
| Zdroj: | Journal of Chromatography A Journal of Chromatography A, Elsevier, 2017, 1487 (11), pp.77-82. ⟨10.1016/j.chroma.2016.11.051⟩ |
| ISSN: | 0021-9673 1873-3778 |
| DOI: | 10.1016/j.chroma.2016.11.051⟩ |
| Popis: | International audience; Countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC) are support free liquid-liquid chromatography techniques sharing the same basic principles and features. Method transfer has previously been demonstrated for both techniques but never from one to another. This study aimed to show such a feasibility using fractionation of Schinus terebinthifolius berries dichloromethane extract as a case study. Heptane - ethyl acetate - methanol -water (6:1:6:1, v/v/v/v) was used as solvent system with masticadienonic and 3β-masticadienolic acids as target compounds. The optimized separation methodology previously described in Part I and II, was scaled up from an analytical hydrodynamic CCC column (17.4mL) to preparative hydrostatic CPC instruments (250mL and 303mL) as a part of method development. Flow-rate and sample loading were further optimized on CPC. Mobile phase linear velocity is suggested as a transfer invariant parameter if the CPC column contains sufficient number of partition cells. |
| Databáze: | OpenAIRE |
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