Molecular characterization of Echinococcus granulosus from Peru by sequencing of the mitochondrial cytochrome C oxidase subunit 1 gene
| Autor: | Octavio Fernandes, Aline C. Volotão, Elizabeth Sánchez, David Garcia, Omar Cáceres, César Náquira, Gladys Patiño, Herrera Silvia |
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| Předmět: |
Microbiology (medical)
Mitochondrial DNA lcsh:Arctic medicine. Tropical medicine Endemic Diseases Genotype Sequence analysis lcsh:RC955-962 Molecular Sequence Data lcsh:QR1-502 Biology Polymerase Chain Reaction lcsh:Microbiology law.invention Electron Transport Complex IV law Echinococcosis genotypes Peru Cytochrome c oxidase Animals Humans Echinococcus granulosus Polymerase chain reaction Phylogeny Genetics Genetic diversity Sheep Molecular epidemiology Base Sequence cytochrome C oxidase subunit 1 DNA Helminth biology.organism_classification Genes Mitochondrial biology.protein Cattle |
| Zdroj: | Europe PubMed Central Scopus-Elsevier Memórias do Instituto Oswaldo Cruz., Vol 105, Iss 6, Pp 806-810 (2010) Memórias do Instituto Oswaldo Cruz, Volume: 105, Issue: 6, Pages: 806-810, Published: SEP 2010 |
| Popis: | Echinococcus granulosus, the etiologic agent of cystic echinococcosis (CE) in humans and other animal species, is distributed worldwide. Ten intra-specific variants, or genotypes (G1-G10), have been defined based on genetic diversity. To determine the genotypes present in endemic areas of Peru, samples were collected from cattle (44), sheep (41) and humans (14) from Junín, Puno Huancavelica, Cusco, Arequipa and Ayacucho. DNA was extracted from protoscolex and/or germinal layers derived from 99 E. granulosus isolates and used as templates to amplify the mitochondrial cytochrome C oxidase subunit 1 gene. The resulting polymerase chain reaction products were sequenced and further examined by sequence analysis. All isolates, independent of the host, exhibited the G1 genotype. Phylogenetic analysis showed that three isolates from Ayacucho shared the same cluster with microvariant G1(4). The G1 genotype is considered the most widespread and infectious form of E. granulosus worldwide and our results confirm that the same patterns apply to this country. Therefore, these findings should be taken into consideration in developing prevention strategies and control programs for CE in Peru. |
| Databáze: | OpenAIRE |
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