Localization of the gene (LAMA4) to chromosome 6q21 and isolation of a partial cDNA encoding a variant laminin A chain
| Autor: | M.A. Leversha, Pope Fm, Carter Np, L. Al-Imara, Allan J. Richards, J. C. Lloyd |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Gene mapping Laminin Complementary DNA Consensus Sequence Genetics medicine Humans Amino Acid Sequence Gene DNA Primers biology medicine.diagnostic_test Base Sequence Molecular Structure DNA–DNA hybridization Nucleic acid sequence Chromosome Mapping Genetic Variation Molecular biology genomic DNA biology.protein Chromosomes Human Pair 6 Fluorescence in situ hybridization |
| Zdroj: | Genomics. 22(1) |
| ISSN: | 0888-7543 |
| Popis: | Laminin is a basement membrane glycoprotein composed of three nonidentical chains, A, B1, and B2. Variant chains such as merosin and S-laminin have been found in different tissues. We have isolated a cDNA encoding a novel laminin A variant that hybridizes to a 6.45-kb mRNA. Using amplification of genomic DNA and flow-sorted chromosomes we have assigned the gene (LAMA4) for this new laminin A variant to chromosome 6. Fluorescence in situ hybridization of a YAC clone further localized the gene to 6q21. |
| Databáze: | OpenAIRE |
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