Development, Characterization and Application of Monoclonal Antibodies against Brazilian Dengue Virus Isolates
| Autor: | Giovanny Augusto Camacho Antevere Mazzarotto, Camila Zanluca, Claudia Nunes Duarte dos Santos, Juliano Bordignon |
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| Rok vydání: | 2014 |
| Předmět: |
Science
viruses Alphavirus Cross Reactions Dengue virus Antibodies Viral medicine.disease_cause Microbiology Virus Cell Line Serology Dengue fever Dengue Epitopes Mice Anticorpos Monoclonais Diagnostic Medicine Virology Medicine and Health Sciences medicine Animals Humans Técnicas e Procedimentos Diagnósticos Multidisciplinary biology business.industry Yellow fever Biology and Life Sciences Antibodies Monoclonal virus diseases Dengue Virus biochemical phenomena metabolism and nutrition medicine.disease biology.organism_classification Immunoglobulin G immunodiagnostic methods Venezuelan equine encephalitis virus Saint Louis encephalitis Medicine Monoclonal antibodies business Brazil Research Article |
| Zdroj: | Repositório Institucional da FIOCRUZ (ARCA) Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ PLoS ONE, Vol 9, Iss 11, p e110620 (2014) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0110620 |
| Popis: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. Dengue is the most prevalent human arboviral disease. The morbidity related to dengue infection supports the need for an early, quick and effective diagnostic test. Brazil is a hotspot for dengue, but no serological diagnostic test has been produced using Brazilian dengue virus isolates. This study aims to improve the development of immunodiagnostic methods for dengue virus (DENV) detection through the production and characterization of 22 monoclonal antibodies (mAbs) against Brazilian isolates of DENV-1, -2 and -3. The mAbs include IgG2bκ, IgG2aκ and IgG1κ isotypes, and most were raised against the envelope or the pre-membrane proteins of DENV. When the antibodies were tested against the four DENV serotypes, different reactivity patterns were identified: group-specific, subcomplex specific (DENV-1, -3 and -4 and DENV-2 and -3) and dengue serotype-specific (DENV-2 or -3). Additionally, some mAbs cross-reacted with yellow fever virus (YFV), West Nile virus (WNV) and Saint Louis encephalitis virus (SLEV). None of the mAbs recognized the alphavirus Venezuelan equine encephalitis virus (VEEV). Furthermore, mAbs D3 424/8G, D1 606/A12/B9 and D1 695/12C/2H were used to develop a capture enzyme-linked immunosorbent assay (ELISA) for anti-dengue IgM detection in sera from patients with acute dengue. To our knowledge, these are the first monoclonal antibodies raised against Brazilian DENV isolates, and they may be of special interest in the development of diagnostic assays, as well as for basic research. |
| Databáze: | OpenAIRE |
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