Cepheid Xpert Xpress Flu/RSV evaluation performed by minimally trained non-laboratory operators in a CLIA-waived environment
| Autor: | Bashar S. Shihabuddin, Matthew L. Faron, Ryan F. Relich, Paul Van Heukelom, Donna Mayne, Mary Allen Staat, Rangaraj Selvarangan, Leslie A. Hueschen, Donna M. Wolk, Stacey House, Glenn Harnett, Kevin McGann, Mark T. Steele, Jose R. Romero, Joe Arms, Owen Lander, Michael Loeffelholz, Fiona Strouts, Daniel Cohen |
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| Rok vydání: | 2022 |
| Předmět: |
Microbiology (medical)
COVID-19 Respiratory Syncytial Virus Infections General Medicine Real-Time Polymerase Chain Reaction Sensitivity and Specificity Influenza B virus Infectious Diseases Molecular Diagnostic Techniques Influenza A virus Nasopharynx Respiratory Syncytial Virus Human Influenza Human Humans Pandemics |
| Zdroj: | Diagnostic Microbiology and Infectious Disease. 104:115764 |
| ISSN: | 0732-8893 |
| DOI: | 10.1016/j.diagmicrobio.2022.115764 |
| Popis: | The COVID-19 pandemic highlighted the significance of readily available and easily performed viral testing for surveillance during future infectious pandemics. The objectives of this study were: to assess the performance of the Xpert Xpress Flu and/or RSV test, a multiplex PCR assay for detecting influenza A and B virus and respiratory syncytial virus nucleic acids in respiratory tract specimens, relative to the Quidel Lyra Influenza A+B assay and the Prodesse ProFlu+ assay, and the system's ease of use by minimally trained operators. Overall, the Xpert Xpress Flu/RSV test demonstrated a high positive and negative percent agreement with the comparator assays, and was easy to use and interpret results, based on the operators' feedback. We concluded that the Xpert Xpress Flu/RSV test is sensitive, specific, and easy to use for the diagnosis of influenza and RSV by minimally trained operators and can be a valuable tool in future infectious clusters or pandemics. |
| Databáze: | OpenAIRE |
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