Bioflocs attenuate Mn-induced bioaccumulation, immunotoxic and oxidative stress via inhibiting GR-NF-κB signalling pathway in Channa asiatica
| Autor: | Lei Zhao, Zhe Yu, Yan-Fen Ma, Yun-Yi Zhao, Ying Zhang, Mu-Yang Li |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fish Proteins
Physiology Health Toxicology and Mutagenesis Aquaculture Pharmacology Toxicology medicine.disease_cause Biochemistry 03 medical and health sciences LSZ reduction formula medicine Animals 030304 developmental biology Inflammation Manganese 0303 health sciences biology Chemistry Fishes 04 agricultural and veterinary sciences Cell Biology General Medicine biology.organism_classification Bioaccumulation Hsp90 Hsp70 Oxidative Stress IκBα Channa asiatica Toxicity 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Oxidative stress |
| Zdroj: | Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 247:109060 |
| ISSN: | 1532-0456 |
| DOI: | 10.1016/j.cbpc.2021.109060 |
| Popis: | Manganese (Mn) is a relatively common element in aquatic ecosystems and can be bio-concentration, but the mechanism of manganese poisoning on fish health is unclear. Here, this study's objective was to evaluate the potential mechanisms of bioflocs in ameliorating Mn-induced toxicity in Channa asiatica. Three hundred sixty juveniles were randomly divided into 12 tanks. Four C:N ratios in triplicate tanks were tried: C/N = 7.6:1 with a commercial diet (control), C/N 10:1, C/N 15:1 and C/N 20:1, and the bio-accumulation, immunotoxic, oxidative stress, GR-NF-κB related genes expression and intestinal histomorphology were assessed in three different periods after Mn exposure (0 h, 48 h and 96 h). The results showed that bioflocs had a significant protective effect on Mn poisoning by preventing alterations in bio-accumulation levels, LSZ, AKP, C3, C4 and IgM, of which the C/N 15:1 group had the best relief effect. Furthermore, bioflocs also assisted in the recovery of liver T-SOD, CAT, GPX and T-AOC levels while decreasing the content of MDA. Moreover, C/N 15:1 group significantly down-regulated the expression level of NF-κB, TNF-α, IL-1β and IL-8 and up-regulated significantly IκBα, GR, HSP70 and HSP90 expression levels considerably (P 0.05). From the intestinal section, the C/N 15:1 group resistance was the best one, and there was no difference between C/N 20:1 group and control group. These results revealed that administration of bioflocs (C/N 15:1) has the potential to combat Mn toxicity in C. asiatica, and the specific pathway may be GR-NF-κB. |
| Databáze: | OpenAIRE |
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