Investigating the Abscopal Effects of Radioablation on Shielded Bone Marrow in Rodent Models Using Multimodality Imaging
| Autor: | Solmaz F. Afshar, Janice A. Zawaski, Omaima M. Sabek, David A. Rendon, M. Waleed Gaber, Arthur W. Zieske, Jyotinder N. Punia, Taeko Inoue |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty Population Biophysics Multimodal Imaging Rats Sprague-Dawley 03 medical and health sciences Mice 0302 clinical medicine Radiation Protection Bone Marrow medicine Animals Radiology Nuclear Medicine and imaging education education.field_of_study Radiation medicine.diagnostic_test business.industry Abscopal effect Magnetic resonance imaging Histology Osteoblast Dose-Response Relationship Radiation Radiotherapy Dosage Bystander Effect Rats Transplantation 030104 developmental biology medicine.anatomical_structure Positron emission tomography 030220 oncology & carcinogenesis Female Bone marrow business |
| Zdroj: | Radiation research. 188(1) |
| ISSN: | 1938-5404 |
| Popis: | The abscopal effect is the response to radiation at sites that are distant from the irradiated site of an organism, and it is thought to play a role in bone marrow (BM) recovery by initiating responses in the unirradiated bone marrow. Understanding the mechanism of this effect has applications in treating BM failure (BMF) and BM transplantation (BMT), and improving survival of nuclear disaster victims. Here, we investigated the use of multimodality imaging as a translational tool to longitudinally assess bone marrow recovery. We used positron emission tomography/computed tomography (PET/CT), magnetic resonance imaging (MRI) and optical imaging to quantify bone marrow activity, vascular response and marrow repopulation in fully and partially irradiated rodent models. We further measured the effects of radiation on serum cytokine levels, hematopoietic cell counts and histology. PET/CT imaging revealed a radiation-induced increase in proliferation in the shielded bone marrow (SBM) compared to exposed bone marrow (EBM) and sham controls. T2-weighted MRI showed radiation-induced hemorrhaging in the EBM and unirradiated SBM. In the EBM and SBM groups, we found alterations in serum cytokine and hormone levels and in hematopoietic cell population proportions, and histological evidence of osteoblast activation at the bone marrow interface. Importantly, we generated a BMT mouse model using fluorescent-labeled bone marrow donor cells and performed fluorescent imaging to reveal the migration of bone marrow cells from shielded to radioablated sites. Our study validates the use of multimodality imaging to monitor bone marrow recovery and provides evidence for the abscopal response in promoting bone marrow recovery after irradiation. |
| Databáze: | OpenAIRE |
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