Mouse mammary tumor gene int-3: a member of the notch gene family transforms mammary epithelial cells
| Autor: | D Gallahan, Robert Callahan, J M Robbins, B J Blondel |
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| Rok vydání: | 1992 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Restriction Mapping Immunology Receptors Cell Surface Sequence alignment Biology Microbiology Cell Line Gene product Mice Mammary Glands Animal Proto-Oncogene Proteins Virology Animals Amino Acid Sequence Receptor Notch4 Gene Peptide sequence Repetitive Sequences Nucleic Acid Mammary tumor Base Sequence Receptors Notch Mouse mammary tumor virus Nucleic acid sequence Membrane Proteins RNA Epithelial Cells Cell Transformation Viral biology.organism_classification Molecular biology Mammary Tumor Virus Mouse Insect Hormones Multigene Family Insect Science DNA Viral Sequence Alignment Research Article |
| Zdroj: | Journal of Virology. 66:2594-2599 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Expression of a 2.3-kb RNA species is induced in mammary tumors as a consequence of insertional mutagenesis at the int-3 locus by the mouse mammary tumor virus. The nucleotide sequence and biological activity of this mammary tumor-specific int-3 RNA species were determined. It contains an open reading frame which encodes a 57-kDa protein. The translated protein possesses six nearly contiguous 32-amino-acid repeats which are related to a similar motif in the Saccharomyces cerevisiae cdc-10-encoded cell cycle protein. In addition, the int-3 cdc-10 repeats are bounded by the PEST amino acid sequence motif which is commonly found in proteins having a rapid turnover and may represent sites for phosphorylation. The int-3 cdc-10 repeat sequences are 50% identical to a portion of the intracellular domain of the neurogenic Drosophila notch gene product. Activation of expression of a recombinant int-3 genomic DNA fragment encoding the 2.3-kb RNA species in HC11 mouse mammary epithelial cells in vitro induces anchorage-independent growth in soft agar. |
| Databáze: | OpenAIRE |
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