Increased specific T cell cytokine responses in patients with active pulmonary tuberculosis from Central Africa
Autor: | Stefan Winkler, Michael Ramharter, Magdalena Necek, Peter G. Kremsner, Ayola A. Adegnika, Heidi Winkler, Thomas Perkmann |
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Rok vydání: | 2005 |
Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Tuberculosis Adolescent CD3 Complex medicine.medical_treatment T cell Immunology Lymphocyte Activation Tuberculin Microbiology Mycobacterium tuberculosis chemistry.chemical_compound Interferon-gamma Immune system Antigen T-Lymphocyte Subsets medicine Humans Gabon Tuberculosis Pulmonary Cells Cultured Aged Brefeldin A biology Tumor Necrosis Factor-alpha Ionomycin Receptors Antigen T-Cell gamma-delta Middle Aged biology.organism_classification medicine.disease Interleukin-10 Infectious Diseases Cytokine medicine.anatomical_structure chemistry Cytokines Interleukin-2 Tumor necrosis factor alpha Female |
Zdroj: | Microbes and infection. 7(9-10) |
ISSN: | 1286-4579 |
Popis: | An understanding of T cell responses that are crucial for control of Mycobacterium tuberculosis (MTB) has major implications for the development of immune-based interventions. We studied the frequency of purified protein derivative (PPD)-specific CD3) cells expressing interleukin-2 (IL)-2, gamma interferon (IFN)-gamma, tumor necrosis factor (TNF)-alpha and IL-10 in HIV-negative pulmonary tuberculosis patients (TB, n=30) as well as in healthy individuals (controls, n=21) from Central Africa. Increased frequencies of PPD-stimulated CD3+ cells expressing IL-2, IFN-gamma, and TNF-alpha in TB were seen when compared with frequencies of controls. The presence of type 1 cytokine biased responses in TB patients was supported by a shift in the distribution pattern of cytokine expression from exclusively IL-2 or TNF-alpha expression seen in controls towards an increased frequency of IFN-gamma/IL-2 or IFN-gamma/TNF-alpha co-expression in TB. Higher levels of PPD-induced IFN-gamma in the supernatants from TB patients than from controls were found, which correlated with its intracellular expression. PPD was a weak inducer of IL-10 in T cells and insufficient in promoting cytokine production in TCRgammadelta+CD3+ cells. Non-specific stimulation with PMA and ionomycin revealed increased frequencies of CD4+ cells expressing IFN-gamma in controls, while expression of IL-2, IL-4, IL-10, IL-13, and TNF-alpha was not different. Non-specific cytokine responses of TCRgammadelta+CD3+ cells were similar in all groups. Pulmonary TB in Central Africa is associated with enhanced expression and secretion of specifically induced cytokines that are frequently implicated in host defense against MTB. |
Databáze: | OpenAIRE |
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