How Molecular Crowding Differs from Macromolecular Crowding: A Femtosecond Mid-Infrared Pump–Probe Study
| Autor: | Minhaeng Cho, Pramod Kumar Verma, Achintya Kundu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
genetic structures
Mid infrared 02 engineering and technology Polyethylene glycol Pump probe 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Crowding 0104 chemical sciences chemistry.chemical_compound chemistry Chemical physics Femtosecond Molecule General Materials Science Physical and Theoretical Chemistry 0210 nano-technology Macromolecular crowding Macromolecule |
| Zdroj: | The Journal of Physical Chemistry Letters. 9:6584-6592 |
| ISSN: | 1948-7185 |
| Popis: | Crowding is an inherent property of living systems in which biochemical processes occur in highly concentrated solutions of various finite-sized species of both low (molecular crowding) and high (macromolecular crowding) molecular weights. Is molecular crowding fundamentally different from macromolecular crowding? To answer this question, we use a femtosecond mid-infrared pump-probe technique with three vibrational probes in molecular (diethylene glycol) and macromolecular (polyethylene glycol) solutions. In less crowded media, both molecular and macromolecular crowders fail to affect the dynamics of interstitial bulk-like water molecules and those at the crowder/water interface. In highly crowded media, interstitial water dynamics strongly depends on molecular crowding, but macromolecular crowding does not alter the bulk-like hydration dynamics and has a modest crowding effect on water at the crowder/water interface. The results of this study provide a molecular level understanding of the structural and dynamic changes to water and the water-mediated cross-linking of crowders. |
| Databáze: | OpenAIRE |
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