Preparation, Characterization And In Vitro Evaluation Of Cisplatin-Bound Triblock Polymeric Micelle Solution For Ovarian Cancer Treatment
| Autor: | Buket Özel, Nur Selvi Günel, Irfan Akartas, H. Y. Karasulu, Ercument Karasulu, Barış Gümüştaş, Güliz Ak, Şenay Hamarat Şanlıer |
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| Rok vydání: | 2021 |
| Předmět: |
System
inorganic chemicals OVCAR-3 Pharmaceutical Science Icp-Ms A2780 Permeability SKOV-3 Biodistribution Drug Discovery medicine neoplasms Platinum Pharmacology Cisplatin Polymeric micelles Chemistry Organic Chemistry polymeric micelle Mononuclear phagocyte system medicine.disease In vitro ovarian cancer Release Biophysics Nanoparticles Ovarian cancer Delivery Nanocarriers medicine.drug |
| Popis: | The main objective of this study was to prepare cisplatin (CDDP) bound triblock polymeric micelle solution which will have a hydrophilic shell not being phagocytosed by mononuclear phagocyte system, and evaluate in vitro behavior for the treatment of ovarian cancer. For this aim, CDDP was bound to polyglutamic acid (PGA) and the triblock polymer was prepared using polyethylene glycol)-polylactide-co-glycolide (PEG-PLGA). CDDP-bound triblock copolymer conjugation was characterized, in vitro release and permeability studies were performed using USP II method and Caco-2 cell lines, respectively. The release of CDDP from CDDP-bound triblock polymeric micelle solution was found 87.3 +/- 3.56% at the end of the 24th hour. CDDP bound triblock polymeric micelle solution was detected as biocompatible, and permeable according to in vitro studies. According to the MTT results, the measured cytotoxicity was found to be maximum in CDDP-bound triblock polymeric micelle solution when compared with CDDP solution and conjugate in SKOV-3 and OVCAR-3 cells, whereas annexin V-FITC apoptosis results were found to be maximum in A2780 cells. Scientific and Technological Research Council of Turkey (TUBITAK) [214S292] This study was financially supported by [The Scientific and Technological Research Council of Turkey (TUBITAK)] under Grant [number 214S292]. |
| Databáze: | OpenAIRE |
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