Changes of extracellular matrix of rat cornea after exposure to hypoxia
| Autor: | Gabriela Mahelkova, J Korynta, A Moravová, J. Wilhelm, R. Vytášek, Jana Novotná |
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| Rok vydání: | 2008 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Free Radicals Physiology Biology Cornea Extracellular matrix chemistry.chemical_compound Internal medicine medicine Animals Corneal Neovascularization Zymography Rats Wistar Eye Proteins Hypoxia Gel electrophoresis chemistry.chemical_classification Analysis of Variance Metalloproteinase Nitrotyrosine General Medicine Hypoxia (medical) Matrix Metalloproteinases Extracellular Matrix Rats Oxidative Stress Endocrinology Enzyme medicine.anatomical_structure chemistry Biochemistry Tyrosine Collagen sense organs medicine.symptom |
| Zdroj: | Physiological Research. :73-80 |
| ISSN: | 1802-9973 0862-8408 |
| DOI: | 10.33549/physiolres.931087 |
| Popis: | The purpose of the study was to check whether hypoxia of corneal tissue increases the collagenolytic activity due to release of reactive oxygen and nitrogen species. Rats were exposed to hypoxia 10 % O2 for 4, 14, and 21 days. The radical tissue injury was measured by the level of nitrotyrosine and changes in the lipoperoxide-related fluorophores. Collagen protein composition was analyzed by slab gel electrophoresis. The activity of gelatinolytic enzymes was studied using the zymography. The vascularization of the corneas was measured. We found no differences in the corneal tissue in the gel electrophoretic profile of collagenous proteins and gelatinolytic activity between normoxic and hypoxic rats. We did not find any sign of radical tissue injury. There were no changes in the vascularization of corneas after exposition to hypoxia. The environmental 10 % hypoxia does not induce radical tissue injury and an increase of collagenolytic activity in the rat cornea. |
| Databáze: | OpenAIRE |
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