Enzymes of collagen synthesis in lung tissues of bleomycin-induced pulmonary fibrosis
Autor: | Prema Palicharla, George C. Fuller, Debayo M. Bolarin |
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Rok vydání: | 1984 |
Předmět: |
Male
Pathology medicine.medical_specialty Pulmonary Fibrosis Procollagen-Proline Dioxygenase Aspartate transaminase Toxicology Bleomycin chemistry.chemical_compound Cricetinae Pulmonary fibrosis medicine Animals Lung Pharmacology chemistry.chemical_classification Mesocricetus integumentary system biology Processing enzymes Lung fibrosis respiratory system Galactosyltransferases medicine.disease Hydroxylysyl galactosyltransferase respiratory tract diseases Enzyme medicine.anatomical_structure chemistry Glucosyltransferases biology.protein Collagen |
Zdroj: | Toxicology and Applied Pharmacology. 73:188-191 |
ISSN: | 0041-008X |
DOI: | 10.1016/0041-008x(84)90066-8 |
Popis: | The activities of prolyl hydroxylase (Pro-OHase), galactosylhydroxylysyl glucosyltransferase (Glu-Gal-Hyl-Tase), and hydroxylysyl galactosyltransferase (Gal-Hyl-Tase) were assayed in lung tissues of hamsters with bleomycin-induced experimental pulmonary fibrosis. Serum Glu-Gal-Hyl-Tase and aspartate transaminase (Asp-NH2-Tase) were measured in the same animals. Lung fibrosis was induced by intratracheal bleomycin instillation, and the enzyme activities were assayed 2, 3, and 4 weeks after bleomycin administration. The activities of the three lung enzymes increased significantly after bleomycin instillation. However, no difference in the values of serum Glu-Gal-Hyl-Tase or Asp-NH2-Tase were observed. Histologic examination of lung sections indicated progressive fibrotic foci. These results thus indicate that the activities of collagen processing enzymes are elevated in the fibrotic lung tissues as a reflection of the increased rate of collagen synthesis during the period of active fibrogenesis, but unlike liver fibrosis, the elevation of tissue Glu-Gal-Hyl-Tase is not predicted by a corresponding increase in serum levels of this enzyme. |
Databáze: | OpenAIRE |
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