Electronic Emulator of Biological Tissue as an Electrical Load during Electroporation
| Autor: | Matej Reberšek, Eva Pirc, Bertrand Balosetti, Damijan Miklavčič |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
electroporation
Electrical load Computer science standard nonlinear load 0206 medical engineering 02 engineering and technology elektroporacija Equivalent impedance transforms lcsh:Technology law.invention sprememba prevodnosti tkiva lcsh:Chemistry 03 medical and health sciences 0302 clinical medicine electroporation emulator law tissue conductivity change Electronic engineering emulator elektroporacije General Materials Science lcsh:QH301-705.5 Instrumentation Fluid Flow and Transfer Processes lcsh:T business.industry biološko breme Process Chemistry and Technology Electroporation standardno nelinearno breme General Engineering 020601 biomedical engineering lcsh:QC1-999 Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 lcsh:TA1-2040 Proof of concept 030220 oncology & carcinogenesis Electrical network Equivalent circuit udc:602.621 lcsh:Engineering (General). Civil engineering (General) business Quality assurance biological load lcsh:Physics Voltage |
| Zdroj: | Applied Sciences Volume 10 Issue 9 Applied Sciences, Vol 10, Iss 3103, p 3103 (2020) Applied sciences, vol. 10, no. 9, 3103, 2020. |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app10093103 |
| Popis: | Electroporation is an emerging technology, with great potential in many different medical and biotechnological applications, food engineering and biomass processing. Large variations of biological load characteristics, however, represent a great challenge in electroporator design, which results in different solutions. Because a clinical electroporator is a medical device, it must comply with medical device regulative and standards. However, none of the existing standards directly address the operation or electroporator&rsquo s performance requirements. In order to evaluate clinical, laboratory and prototype electroporation devices during the development process, or to evaluate their final performance considering at least from the perspective of output pulse parameters, we present a case study on the design of an electronic emulator of biological tissue as an electrical load during electroporation. The proposed electronic load emulator is a proof of concept, which enables constant and sustainable testing and unbiased comparison of different electroporators&rsquo operations. We developed an analog electrical circuit that has equivalent impedance to the beef liver tissue in combination with needle electrodes, during high voltage pulse delivery and/or electroporation. Current and voltage measurements during electroporation of beef liver tissue ex vivo, were analyzed and parametrized to define the analog circuit equation. An equivalent circuit was simulated, built and validated. The proposed concept of an electronic load emulator can be used for &ldquo classical&rdquo electroporator (i.e., not nanosecond) performance evaluation and comparison of their operation. Additionally, it facilitates standard implementation regarding the testing protocol and enables quality assurance. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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