Bioactive glass‐containing hydrogel delivery system for osteogenic differentiation of human dental pulp stem cells
| Autor: | Faezeh Shahnazi, Sahar Ansari, Chider Chen, Sevda Pouraghaei Sevari, Alireza Moshaverinia, John C. Mitchell |
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| Rok vydání: | 2019 |
| Předmět: |
Ceramics
Materials science 0206 medical engineering Biomedical Engineering Biocompatible Materials 02 engineering and technology law.invention Biomaterials Tissue engineering Osteogenesis law Dental pulp stem cells Humans Bone regeneration Cells Cultured Dental Pulp Matrigel Stem Cells technology industry and agriculture Metals and Alloys Cell Differentiation Hydrogels 021001 nanoscience & nanotechnology 020601 biomedical engineering Cell biology Bioactive glass Self-healing hydrogels Ceramics and Composites Delivery system Stem cell 0210 nano-technology |
| Zdroj: | Journal of Biomedical Materials Research Part A. 108:557-564 |
| ISSN: | 1552-4965 1549-3296 |
| DOI: | 10.1002/jbm.a.36836 |
| Popis: | Osteoconductive hydrogels can be fabricated by incorporating necessary growth factors and bioactive particles or simply utilizing the ability of the hydrogel itself to induce bone regeneration. The osteogenic inductive potential of the bioactive glass microparticles (BG MPs) has been well-studied. However, the role of the hydrogel embedding the BG MPs on the osteogenic differentiation of the encapsulated stem cells has not been well established. Moreover, it has been reported that the dental pulp stem cell (DPSC) has the capability of regenerating the craniofacial bone tissue when receiving the necessary osteogenic signals from the microenvironment. In the current study, we have fabricated a composite hydrogel based on alginate and Matrigel containing BG MPs and evaluated the role of the BG MPs and Matrigel on osteogenic differentiation of the encapsulated DPSCs. Our results confirmed that presence of Matrigel enhances the osteogenic differentiation of the DPSCs regardless of the decrease in elasticity of the hydrogel in presence of the BG MPs. This strategy of modifying the microenvironment can be a promising approach for craniofacial bone tissue engineering. |
| Databáze: | OpenAIRE |
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