Characterization of isolates of Listeria monocytogenes from sludge using pulsed-field gel electrophoresis and virulence assays
| Autor: | Sylvie M. Roche, M. Marault, N. Garrec, Anne Brisabois, M. Federighi, Anne-Marie Pourcher, Philippe Velge, A. Kerouanton |
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| Přispěvatelé: | Agence Française de Sécurité Sanitaire des Aliments (AFSSA), Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Laboratoire des Sciences de l'Environnement et de l'Aménagement (LSEA), LSEA, SECurité des ALIments et Microbiologie, Institut National de la Recherche Agronomique (INRA)-École nationale d'ingénieurs des techniques des industries agricoles et alimentaires (ENITIAA)-Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), French Environment and Energy Agency (ADEME), Gestion environnementale et traitement biologique des déchets (UR GERE), Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF), Ecole Nationale Vétérinaire de Nantes, Université d'Angers (UA), Garrec, Nathalie |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Serotype
VIRULENCE ASSAY [SDV]Life Sciences [q-bio] Virulence Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology 03 medical and health sciences Mice Listeria monocytogenes Pulsed-field gel electrophoresis medicine Bioassay Animals Humans Serotyping Pathogen 030304 developmental biology SLUDGE 2. Zero hunger 0303 health sciences Sewage 030306 microbiology boue PFGE SUBTYPING General Medicine biology.organism_classification 3. Good health Electrophoresis Gel Pulsed-Field Molecular Typing [SDE]Environmental Sciences ISOLATE CHARACTERIZATION Mouse virulence Bacteria Biotechnology |
| Zdroj: | Journal of Applied Microbiology Journal of Applied Microbiology, Wiley, 2010, 108 (4), pp.1380-1388. ⟨10.1111/j.1365-2672.2009.04531.x⟩ Journal of Applied Microbiology 4 (108), 1380-1388. (2010) |
| ISSN: | 1364-5072 1365-2672 |
| DOI: | 10.1111/j.1365-2672.2009.04531.x⟩ |
| Popis: | International audience; Aims : To study the diversity and virulence of Listeria monocytogenes isolated from sludge. Methods and results : A total of 60 isolates of L. monocytogenes from sludge were characterized by serotyping, PFGE typing and using in vitro and in vivo virulence assays. The PFGE patterns were compared with those of food and human isolates to determine whether specific group clones are associated with environmental samples. The 60 isolates gave 44 different combined ApaI/AscI PFGE patterns. The PFGE patterns of most isolates were similar or very similar to those of epidemic isolates. The majority (93%) of isolates were found to be virulent by plaque-forming assay and by mouse virulence assay. Conclusions : Our findings suggest that L. monocytogenes strains found in non-sanitized sludge are virulent and represent a potential health hazard. Although no case of listeriosis related to sludge spread onto agricultural land has been reported, particular attention to this pathogen is needed. Significance and Impact of the study: This is the first study dealing with the characterization of L. monocytogenes isolates from non-sanitized sludge samples by molecular typing methods and in vitro and in vivo virulence assays. Our findings provide relevant information for evaluating the health risks associated with spreading sludge onto agricultural land. |
| Databáze: | OpenAIRE |
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