Fungal lysozyme leverages the gut microbiota to curb DSS-induced colitis
| Autor: | André Marette, Benjamin A. H. Jensen, Erica Bonazzi, Lea Benedicte Skov Hansen, Esben Gjerløff Wedebye Schmidt, Torsten Schröder, Christian Sina, Ida S. Larsen, Laurence Morin, Benoit Chassaing, Peter Bjarke Olsen, Annika Suenderhauf, Nanna N. Kristensen, Béatrice S.-Y. Choi |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Fasting hyperinsulinemia colitis RC799-869 Gut flora Mice chemistry.chemical_compound 0302 clinical medicine Oral administration insulin resistance intestinal inflammation Gastrointestinal Microbiome/drug effects Colitis/chemically induced 2. Zero hunger Mice Inbred BALB C 0303 health sciences biology host defense peptides Dextran Sulfate high fat diet Gastroenterology Diseases of the digestive system. Gastroenterology Acremonium Infectious Diseases Female 030211 gastroenterology & hepatology medicine.symptom Lysozyme Bacteria/classification Research Article Research Paper Microbiology (medical) Inflammation muramidase Microbiology digestive system 03 medical and health sciences Insulin resistance mucus microbiota encroachment medicine Animals Humans Microbiome Colitis 030304 developmental biology Bacteria medicine.disease biology.organism_classification gut health Gastrointestinal Microbiome Mice Inbred C57BL Disease Models Animal chemistry Immunology Dextran Sulfate/adverse effects microbiota function Gut health Muramidase/administration & dosage Acremonium/enzymology |
| Zdroj: | Gut Microbes, Vol 13, Iss 1 (2021) Larsen, I S, Jensen, B A H, Bonazzi, E, Choi, B S Y, Kristensen, N N, Schmidt, E G W, Süenderhauf, A, Morin, L, Olsen, P B, Hansen, L B S, Schröder, T, Sina, C, Chassaing, B & Marette, A 2021, ' Fungal lysozyme leverages the gut microbiota to curb DSS-induced colitis ', Gut Microbes, vol. 13, no. 1, 1988836 . https://doi.org/10.1080/19490976.2021.1988836 Larsen, I S, Jensen, B A H, Bonazzi, E, Choi, B S Y, Kristensen, N N, Schmidt, E G W, Morin, L, Olsen, P B, Hansen, L B S, Schröder, T, Sina, C, Chassaing, B, Marette, A & Sünderhauf, A 2021, ' Fungal lysozyme leverages the gut microbiota to curb DSS-induced colitis ', Gut Microbes, vol. 13, no. 1, pp. 1988836 . https://doi.org/10.1080/19490976.2021.1988836 Gut Microbes article-version (VoR) Version of Record |
| ISSN: | 1949-0984 1949-0976 |
| DOI: | 10.1080/19490976.2021.1988836 |
| Popis: | Colitis is characterized by colonic inflammation and impaired gut health. Both features aggravate obesity and insulin resistance. Host defense peptides (HDPs) are key regulators of gut homeostasis and generally malfunctioning in above-mentioned conditions. We aimed here to improve bowel function in diet-induced obesity and chemically induced colitis through daily oral administration of lysozyme, a well-characterized HDP, derived from Acremonium alcalophilum. C57BL6/J mice were fed either low-fat reference diet or HFD ± daily gavage of lysozyme for 12 weeks, followed by metabolic assessment and evaluation of colonic microbiota encroachment. To further evaluate the efficacy of intestinal inflammation, we next supplemented chow-fed BALB/c mice with lysozyme during Dextran Sulfate Sodium (DSS)-induced colitis in either conventional or microbiota-depleted mice. We assessed longitudinal microbiome alterations by 16S amplicon sequencing in both models. Lysozyme dose-dependently alleviated intestinal inflammation in DSS-challenged mice and further protected against HFD-induced microbiota encroachment and fasting hyperinsulinemia. Observed improvements of intestinal health relied on a complex gut flora, with the observation that microbiota depletion abrogated lysozyme’s capacity to mitigate DSS-induced colitis. Akkermansia muciniphila associated with impaired gut health in both models, a trajectory that was mitigated by lysozyme administration. In agreement with this notion, PICRUSt2 analysis revealed specific pathways consistently affected by lysozyme administration, independent of vivarium, disease model and mouse strain. Taking together, lysozyme leveraged the gut microbiota to curb DSS-induced inflammation, alleviated HFD-induced gastrointestinal disturbances and lowered fasting insulin levels in obese mice. Collectively, these data present A. alcalophilum-derived lysozyme as a promising candidate to enhance gut health. |
| Databáze: | OpenAIRE |
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