Changes in the epigenome and transcriptome of the poplar shoot apical meristem in response to water availability affect preferentially hormone pathways
| Autor: | Clément Lafon-Placette, Alain Delaunay, Anne-Laure Le Gac, David Cohen, Marie-Claude Lesage-Descauses, Béline Jesson, Stéphane Maury, Franck Brignolas, Didier Chauveau, Irène Hummel, Didier Le Thiec, Vincent Segura, Marie-Béatrice Bogeat-Triboulot |
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| Přispěvatelé: | Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), Institut Denis Poisson (IDP), Centre National de la Recherche Scientifique (CNRS)-Université de Tours (UT)-Université d'Orléans (UO), Biologie intégrée pour la valorisation de la diversité des arbres et de la forêt (BioForA), Institut National de la Recherche Agronomique (INRA)-Office National des Forêts (ONF), Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), IMAXIO, ANR (GPLA06028G, ANR-12-LABXARBRE-01), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Université d'Orléans (UO) |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology [SDV]Life Sciences [q-bio] Meristem Plant Science Biology water availability 01 natural sciences phenotypic plasticity Populus×euramericana Epigenesis Genetic Transcriptome 03 medical and health sciences Plant Growth Regulators Epigenetics Gene 2. Zero hunger shoot apical meristem Phenotypic plasticity DNA methylation Water Epigenome 15. Life on land Cell biology differentially methylated regions 030104 developmental biology Differentially methylated regions Populus Differentially expressed genes Genome Plant Plant Shoots 010606 plant biology & botany Signal Transduction |
| Zdroj: | Journal of Experimental Botany Journal of Experimental Botany, Oxford University Press (OUP), 2018, 69 (3), pp.537-551. ⟨10.1093/jxb/erx409⟩ |
| ISSN: | 0022-0957 1460-2431 |
| DOI: | 10.1093/jxb/erx409⟩ |
| Popis: | International audience; The adaptive capacity of long-lived organisms such as trees to the predicted climate changes, including severe and successive drought episodes, will depend on the presence of genetic diversity and phenotypic plasticity. Here, the involvement of epigenetic mechanisms in phenotypic plasticity toward soil water availability was examined in Populus×euramericana. This work aimed at characterizing (i) the transcriptome plasticity, (ii) the genome-wide plasticity of DNA methylation, and (iii) the function of genes affected by a drought-rewatering cycle in the shoot apical meristem. Using microarray chips, differentially expressed genes (DEGs) and differentially methylated regions (DMRs) were identified for each water regime. The rewatering condition was associated with the highest variations of both gene expression and DNA methylation. Changes in methylation were observed particularly in the body of expressed genes and to a lesser extent in transposable elements. Together, DEGs and DMRs were significantly enriched in genes related to phytohormone metabolism or signaling pathways. Altogether, shoot apical meristem responses to changes in water availability involved coordinated variations in DNA methylation, as well as in gene expression, with a specific targeting of genes involved in hormone pathways, a factor that may enable phenotypic plasticity. |
| Databáze: | OpenAIRE |
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