Two immunodominant domains of gp41 bind antibodies which enhance human immunodeficiency virus type 1 infection in vitro
| Autor: | M. K. Gorny, S. Zolla-Pazner, William M. Mitchell, W E Robinson, Jian-Yin Xu |
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| Rok vydání: | 1991 |
| Předmět: |
medicine.drug_class
Molecular Sequence Data Immunology Dose-Response Relationship Immunologic Enzyme-Linked Immunosorbent Assay Peptide HIV Antibodies Monoclonal antibody Gp41 Binding Competitive Microbiology Virus Cell Line Viral envelope Virology medicine Humans Amino Acid Sequence Peptide sequence chemistry.chemical_classification biology Antibodies Monoclonal virus diseases Complement System Proteins Molecular biology HIV Envelope Protein gp41 Peptide Fragments chemistry Polyclonal antibodies Insect Science HIV-1 biology.protein Antibody Research Article |
| Zdroj: | Journal of Virology. 65:4169-4176 |
| ISSN: | 1098-5514 0022-538X |
| Popis: | Four of eight human monoclonal antibodies (huMAbs) to gp41 were identified which could enhance human immunodeficiency virus type 1 (HIV-1) infection in vitro by complement-mediated antibody-dependent enhancement (C'-ADE). These enhancing huMAbs were mapped to two distinct domains on the HIV-1 gp41 transmembrane glycoprotein by using synthetic peptides. The first domain, amino acids 579 to 613 (peptide AA579-613), was recognized by three of the four enhancing huMAbs. The AA579-613 peptide blocked C'-ADE of HIV-1 infection in vitro whether it was mediated by these three huMAbs or by human polyclonal anti-HIV serum. The second domain, amino acids 644 to 663, bound the remaining enhancing huMAb. This peptide weakly blocked C'-ADE mediated by the huMAb and by an HIV immune globulin fraction but did not block C'-ADE mediated by a patient's serum. The patient's serum did react with the peptide in an enzyme immunoassay. The huMAbs to the two domains could interact in vitro to enhance HIV-1 infection in a synergistic manner. These two domains, which bind enhancing antibodies, are conserved between HIV-1 isolates as well as between HIV-2 and simian immunodeficiency virus isolates. These data demonstrate the existence of two conserved regions within the HIV-1 gp41 which bind enhancing antibodies; these two domains, amino acids 579 to 613 and 644 to 663, may prove important in HIV-1 vaccine development and in immunopathogenesis of HIV-1 infection. |
| Databáze: | OpenAIRE |
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