Graphene oxide - gelatin nanohybrids as functional tools for enhanced Carboplatin activity in neuroblastoma cells
Autor: | Bernd Büchner, Elizabeth Hinde, Silke Hampel, Giuseppe Cirillo, Maria Kavallaris, Steffen Oswald, Michael Mertig, Sami Makharza, Orazio Vittorio |
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Rok vydání: | 2014 |
Předmět: |
food.ingredient
Cell Survival Chemistry Pharmaceutical Oxide Pharmaceutical Science Nanoparticle Nanotechnology Antineoplastic Agents Microscopy Atomic Force Spectrum Analysis Raman Gelatin law.invention Carboplatin chemistry.chemical_compound Neuroblastoma food law Cell Line Tumor medicine Humans Technology Pharmaceutical Pharmacology (medical) neoplasms health care economics and organizations Pharmacology Drug Carriers Dose-Response Relationship Drug Graphene Photoelectron Spectroscopy Organic Chemistry Oxides medicine.disease Combinatorial chemistry Nanomedicine chemistry Microscopy Electron Scanning Molecular Medicine Nanoparticles Graphite Spectrophotometry Ultraviolet Drug carrier Biotechnology |
Zdroj: | Pharmaceutical research. 32(6) |
ISSN: | 1573-904X |
Popis: | Preparation of Nanographene oxide (NGO) - Gelatin hybrids for efficient treatment of Neuroblastoma.Nanohybrids were prepared via non-covalent interactions. Spectroscopic tools have been used to discriminate the chemical states of NGO prior and after gelatin coating, with UV visible spectroscopy revealing the maximum binding capacity of gelatin to NGO. Raman and X-ray photoelectron spectroscopy (XPS) demonstrated NGO and Gelatin_NGO nanohybrids through a new chemical environments produced after noncovalent interaction. Microscopic analyses, atomic force microscopy (AFM) and scanning electron microscopy (SEM) are used to estimate the thickness of samples and the lateral width in the nanoscale, respectively.The cell viability assay validated Gelatin_NGO nanohybrids as a useful nanocarrier for Carboplatin (CP) release and delivery, without obvious signs of toxicity. The nano-sized NGO (200 nm and 300 nm) did not enable CP to kill the cancer cells efficiently, whilst the CP loaded Gel_NGO 100 nm resulted in a synergistic activity through increasing the local concentration of CP inside the cancer cells.The nanohybrids provoked high stability and dispersibility in physiological media, as well as enhanced the anticancer activity of the chemotherapy agent Carboplatin (CP) in human neuroblastoma cells. |
Databáze: | OpenAIRE |
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