Plasma Chemokine signature correlates with lung goblet cell hyperplasia in smokers with and without chronic obstructive pulmonary disease
| Autor: | Gerard J. Criner, William K. Cornwell, Victor Kim, Heba Y Durra Md, Michelle Oros, Thomas J. Rogers |
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| Jazyk: | angličtina |
| Předmět: |
Male
Vascular Endothelial Growth Factor A Pathology Macrophage medicine.medical_treatment Systemic inflammation Gastroenterology Pulmonary Disease Chronic Obstructive Chemokine CCL7 Chemokine CCL4 Lung Chemokine CCL2 Chemokine CCL3 COPD Chronic obstructive pulmonary disease Neutrophil Interleukin-17 Smoking Hyperplasia Middle Aged 3. Good health medicine.anatomical_structure Cytokine Chemokine Cytokines Female Interleukin 17 Goblet Cells medicine.symptom Chemokines Research Article Adult Pulmonary and Respiratory Medicine medicine.medical_specialty Internal medicine medicine Humans Interleukin 9 Aged Chemokine CCL22 Epidermal Growth Factor business.industry Interleukin-6 Interleukin-8 Case-control study Interleukin-9 Mucins medicine.disease respiratory tract diseases Case-Control Studies Mucin Interleukin-4 business Goblet cell hyperplasia Interleukin-1 |
| Zdroj: | BMC Pulmonary Medicine |
| ISSN: | 1471-2466 |
| DOI: | 10.1186/s12890-015-0103-2 |
| Popis: | Background Chronic Obstructive Pulmonary Disease (COPD) is characterized by lung and systemic inflammation as well as airway goblet cell hyperplasia (GCH). Mucin production is activated in part by stimulation of the epidermal growth factor (EGF) receptor pathway through neutrophils and macrophages. How circulating cytokine levels relate to GCH is not clear. Methods We performed phlebotomy and bronchoscopy on 25 subjects (six nonsmokers, 11 healthy smokers, and eight COPD subjects FEV1 30–60 %). Six endobronchial biopsies per subject were performed. GCH was measured by measuring mucin volume density (MVD) using stereological techniques on periodic acid fast-Schiff stained samples. We measured the levels of chemokines CXCL8/IL-8, CCL2/MCP-1, CCL7/MCP-3, CCL22/MCD, CCL3/MIP-1α, and CCL4/MIP-1β, and the cytokines IL-1, IL-4, IL-6, IL-9, IL-17, EGF, and vascular endothelial growth factor (VEGF). Differences between groups were assessed using one-way ANOVA, t test, or Chi squared test. Post hoc tests after ANOVA were performed using Bonferroni correction. Results MVD was highest in healthy smokers (27.78 ± 10.24 μL/mm2) compared to COPD subjects (16.82 ± 16.29 μL/mm2, p = 0.216) and nonsmokers (3.42 ± 3.07 μL/mm2, p |
| Databáze: | OpenAIRE |
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