A Synthetic Circuit for Mercury Bioremediation Using Self-Assembling Functional Amyloids
| Autor: | Neel Joshi, Pei Kun Richie Richie Tay, Peter Q. Nguyen |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Amyloid Mercury pollution 030106 microbiology Biomedical Engineering chemistry.chemical_element Biology Protein Engineering Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences Synthetic biology Bioremediation Self assembling business.industry Sustainable strategy fungi Biofilm food and beverages Mercury General Medicine Biotechnology Mercury (element) Biodegradation Environmental 030104 developmental biology chemistry Biofilms Synthetic Biology Biochemical engineering business |
| Zdroj: | ACS Synthetic Biology. 6:1841-1850 |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/acssynbio.7b00137 |
| Popis: | Synthetic biology approaches to bioremediation are a key sustainable strategy to leverage the self-replicating and programmable aspects of biology for environmental stewardship. The increasing spread of anthropogenic mercury pollution into our habitats and food chains is a pressing concern. Here, we explore the use of programmed bacterial biofilms to aid in the sequestration of mercury. We demonstrate that by integrating a mercury-responsive promoter and an operon encoding a mercury-absorbing self-assembling extracellular protein nanofiber, we can engineer bacteria that can detect and sequester toxic Hg2+ ions from the environment. This work paves the way for the development of on-demand biofilm living materials that can operate autonomously as heavy-metal absorbents. |
| Databáze: | OpenAIRE |
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